Detection of synthetic cannabinoids
View Patent ↗The invention describes methods and kits for detecting and determining current and future synthetic cannabinoids from the JWH and RCS families. Unique antibodies derived from novel immunogens enable said methods and kits.
1. An antibody which binds to:
wherein R 5,1 is a C 2 -C 5 substituted or unsubstituted hydrocarbon chain; and R 5,2 is selected from the group consisting of methoxy, CH 3 and chlorine;
wherein the antibody has a cross-reactivity presented as B/B 0 of ≦16%, standardised with RCS-8 as standard, and using a tracer ESC6557, against each one of the cross-reactants selected from the group consisting of JWH-203, JWH-250, JWH-250 N-(5-hydroxypentyl) metabolite, JWH-250 N-(5-carboxypentyl) metabolite, JWH-251 and RCS-8 (SR-18) at a cross-reactant concentration of 100 ng/ml,
wherein RCS-8 is
wherein ESC6557 is
wherein Mal is maleimide,
wherein HRP is horse radish peroxidase,
wherein B 0 is absorbance at 450 nm at zero ng/ml standard concentration, and
wherein B is absorbance at 450 nm at predetermined variable standard concentrations.
2. The antibody of claim 1 wherein R 5,1 is selected from the group consisting of substituted ethyl; substituted butyl; pentyl; and substituted pentyl.
3. The antibody of claim 2 wherein R 5,1 is selected from the group consisting of 2-ethyl-cyclohexyl; butyryl; pentyl; and 5-hydroxypentyl.
4. The antibody of claim 1 , wherein the antibody is raised from Immunogen V:
wherein C 4 is a C 4 hydrocarbon chain and BTG is bovine thyroglobulin.
5. A kit for detecting or determining at least one selected from the group consisting of at least one molecule of the JWH and RCS families and at least one metabolite thereof, the kit comprising the antibody of claim 1 .