Detection of synthetic cannabinoids
View Patent ↗The invention describes methods and kits for detecting and determining current and future synthetic cannabinoids from the JWH and CP families. Unique antibodies derived from novel immunogens enable said methods and kits.
1. An antibody that binds to structure:
wherein
R 4,1 is a C 4 or C 5 , substituted or unsubstituted, saturated or unsaturated, hydrocarbon chain; and,
Y is substituted or unsubstituted naphthyl or substituted phenyl; and,
wherein the antibody has a cross-reactivity presented as B/B 0 % of ≦15% (standardised with JWH-018 and using tracer ESC5913,
wherein JWH-018 is
wherein ESC5913 is
and
wherein HRP is Horse Radish Peroxidase)
against each one of the cross-reactants selected from the group consisting of JWH-018, JWH-018 (N-(3-methylbutyl) isomer, JWH-073, JWH-073 4-methylnaphthyl analogue, JWH-022, JWH-081, JWH-081 5-methoxynaphthyl isomer, JWH-122, JWH-122 6-methylnaphthyl isomer, JWH-122 7-methylnaphthyl isomer, JWH-182, JWH-210, JWH-398, JWH-398 5-chloronaphthyl isomer, AM-694, AM-2201, and AM-2201 N-(4-fluoropentyl) isomer,
at a cross-reactant concentration of 100 ng/ml,
wherein B 0 is absorbance at 450 nm at zero ng/ml standard concentration, and
wherein B is absorbance at 450 nm at predetermined standard concentrations.