Firefly luciferase
According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.
1. A luciferase comprising an amino acid sequence represented by SEQ ID NO: 1.
2. The luciferase according to claim 1 , wherein the amino acid sequence comprises at least one substitution of a group consisting of:
D438G substitution in which an amino acid corresponding to an aspargic acid at position 438 of the amino acid sequence of SEQ ID NO: 1 is glycine;
I532R substitution in which an amino acid corresponding to an isoleucine at position 532 of the amino acid sequence of SEQ ID NO: 1 is arginine;
E356R substitution in which an amino acid corresponding to a glutamic acid at position 356 of the amino acid sequence of SEQ ID NO: 1 is arginine; and
V368A substitution in which an amino acid corresponding to a valine at position 368 of the amino acid sequence of SEQ ID NO: 1 is alanine.
3. The luciferase according to claim 1 , wherein an amino acid corresponding to an aspargic acid at position 438 of the amino acid sequence of SEQ ID NO: 1 is glycine,
an amino acid corresponding to an isoleucine at position 532 of the amino acid
an amino acid corresponding to a glutamic acid at position 356 of the amino acid sequence of SEQ ID NO: 1 is arginine.
4. The luciferase according to claim 1 , wherein an amino acid corresponding to a glutamic acid at position 356 of the amino acid sequence of SEQ ID NO: 1 is arginine, and
an amino acid correspoding to a valine at position 368 of the amino acid sequence of SEQ ID NO: 1 is alanine.