Kits comprising linear DNAs for sustained polypeptide expression using synthetic, modified RNAs
Described herein are synthetic, modified RNAs for changing the phenotype of a cell, such as expressing a polypeptide or altering the developmental potential. Accordingly, provided herein are compositions, methods, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells. These methods, compositions, and kits comprising synthetic, modified RNAs can be used either to express a desired protein in a cell or tissue, or to change the differentiated phenotype of a cell to that of another, desired cell type.
1. A kit for making synthetic mRNAs for the production of induced pluripotent stem (iPS) cells comprising the following components:
(a) a linear DNA template for the generation of a synthetic modified RNA, the template encoding an OCT4 mRNA of SEQ ID NO: 788;
(b) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a SOX2 mRNA of SEQ ID NO: 941 or SEQ ID NO: 1501;
(c) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a KLF4 mRNA of SEQ ID NO: 501;
(d) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a c-MYC mRNA of SEQ ID NO: 636; and
(e) a ribonucleoside mixture component comprising 5-methylcytidine and pseudouridine.
2. The kit of claim 1 , wherein a T7 promoter is operably linked upstream of each of the linear DNAs encoding the OCT4 mRNA, SOX2 mRNA, KLF4 mRNA, and c-MYC mRNA.
3. The kit of claim 1 , wherein the ribonucleoside mixture further comprises a 5′ cap analog nucleoside component.
4. The kit of claim 3 , wherein the 5′ cap analog is 3″-0-Me-m 7 G(5′)ppp(5′)G.
5. The kit of claim 1 , further comprising a phosphatase enzyme, an RNA polymerase enzyme, or both.
6. The kit of claim 1 , further comprising a buffer.
7. The kit of claim 1 , further comprising a linear DNA template for the generation of a synthetic modified RNA, the template encoding a sequence encoding a soluble interferon receptor.
8. The kit of claim 7 , wherein the soluble interferon receptor is B18R.