Branched polymers
View Patent ↗The present invention is directed to branched reactive water-soluble polymers comprising at least two polymer arms, such as poly(ethylene glycol), attached to a central aliphatic hydrocarbon core molecule through ether linkages. The branched polymers bear at least one functional group for reacting with a biologically active agent to form a biologically active conjugate. The functional group of the branched polymer can be directly attached to the aliphatic hydrocarbon core or via an intervening linkage, such as a heteroatom, -alkylene-, —O-alkylene-O—, -alkylene-O-alkylene-, -aryl-O—, —O-aryl-, (—O-alkylene-) m , or (-alkylene-O—) m linkage, wherein m is 1-10.
1. A method of preparing a biologically active conjugate of the formula
D-L 1 -(X) p -R(—X′-POLY) q
comprising:
reacting a branched reactive polymer having the structure Y—(X)p-R(—X′-POLY) q with a biologically active molecule D;
wherein:
Y is a functional group;
R is an aliphatic hydrocarbon having a length of at least three carbon atoms;
X′ is —O—;
X is a linker of 1 to 10 atoms in length;
p is 0 or 1;
q is 2 to about 10;
L 1 is a linkage resulting from the reaction of a functional group on the linker (X), when present, or on the aliphatic hydrocarbon having a length of at least three carbon atoms (R) of the branched polymer and a functional group of the biologically active molecule; and
each POLY is a water soluble and non-peptidic polymer that terminates with a hydroxyl or methoxy group, and further wherein the branched polymer has a molecular weight of about 12,000 Da to about 100,000 Da.
2. The method of claim 1 , wherein D is selected from the group consisting of peptides, proteins, enzymes, drugs, dyes, lipids, nucleosides, nucleotides, oligonucleotides, polynucleotides, nucleic acids, polysaccharides, steroids, cells, viruses, liposomes, micelles, and fats.
3. The method of claim 1 , wherein each water soluble and non-peptidic polymer is a PEG.
4. The method of claim 1 , wherein each water soluble and non-peptidic polymer is a PEG that terminates with a hydroxyl group.
5. The method of claim 1 , wherein each water soluble and non-peptidic polymer is a PEG that terminates with a methoxy group.
6. The method of claim 1 , wherein Y selected from the group consisting of hydroxyl, active ester, active carbonate, acetal, aldehyde, aldehyde hydrate, alkenyl, acrylate, methacrylate, acrylamide, active sulfone, amine, hydrazide, thiol, alkanoic acid, acid halide, isocyanate, isothiocyanate, maleimide, vinylsulfone, dithiopyridine, vinylpyridine, iodoacetamide, epoxide, glyoxal, dione, mesylate, tosylate, and tresylate.
7. The method of claim 1 , wherein p is 1 and X is selected from the group consisting of a heteroatom, -alkylene-, —O-alkylene-O—, -alkyl ene-O-alkylene-, -aryl-O—, —O-aryl-, (—O— alkylene-) m , and (-alkylene-O—) m , wherein m is 1-10.
8. The method of claim 1 , wherein p is 0 and Y is hydroxyl.
9. The method of claim 1 , wherein the branched polymer has a molecular weight of about 20,000 Da.
10. The method of claim 1 , wherein the branched polymer has a molecular weight of about 40,000 Da.
11. The method of claim 1 , wherein the branched polymer has a molecular weight of about 60,000 Da.
12. The method of claim 1 , wherein the biologically active conjugate has the structure: