Allogeneic cell compositions with cross-linked CD3/CD28
A method of manipulating allogeneic cells for use in allogeneic cell therapy providing a composition of highly activated allogeneic T-cells which are infused into immunocompetent cancer patients to elicit a novel anti-tumor immune mechanism, or “Mirror Effect”. In contrast to current allogeneic cell therapy protocols where T-cells in the graft mediate the beneficial graft vs. tumor (GVT) and detrimental graft vs. host (GVH) effects, the allogeneic cells of the present invention stimulate host T-cells to mediate the “mirror” of these effects. The mirror of the GVT effect is the host vs. tumor (HVT) effect. The “mirror” of the GVH effect is the host vs. graft (HVG) effect. The anti-tumor HVT effect occurs in conjunction with a non-toxic HVG rejection effect. The highly activated allogeneic cells of the invention can be used to stimulate host immunity in a complete HLA mis-matched setting in a patient.
1. A composition comprising at least a portion of T-cells, the T-cells being derived from a healthy donor and being in a state of activation due to CD3/CD28 crosslinking and said T-cells being suspended in a media suitable for human administration for therapy.
2. The composition of claim 1 wherein the T-cells are predominately CD4+ cells.
3. The composition of claim 2 wherein the CD4+ cells are predominately Th1 cells.
4. The composition of claim 1 wherein the cells are suspended in a syringe.
5. The composition of claim 1 wherein the cells are suspended in a collapsible container.