Microfluidic apparatus and control method thereof
A microfluidic apparatus having an additional chamber containing material configured to prevent cross contamination between reaction chambers contained therein, and a control method thereof are provided. The microfluidic apparatus includes a sample chamber configured to accommodate a sample, a plurality of reaction chambers each configured to accommodate a reagent, a distribution channel configured to distribute the sample into the plurality of reaction chambers, a mixture prevention chamber connected to the distribution channel and containing a mixture prevention material configured to prevent the reagents accommodated in the plurality of reaction chambers from being mixed with each other, and a valve disposed within the distribution channel and configured to open and close the distribution channel.
1. A microfluidic apparatus comprising:
a sample chamber configured to accommodate a sample;
a plurality of reaction chambers each configured to accommodate a reagent;
a distribution channel configured to distribute the sample from the sample chamber into the plurality of reaction chambers;
a mixture prevention chamber connected to the distribution channel and containing a mixture prevention material configured to prevent the reagents accommodated in the plurality of reaction chambers from being mixed with each other; and
at least one valve disposed within the distribution channel and configured to open and close the distribution channel.
2. The microfluidic apparatus of claim 1 , wherein the at least one valve comprises a first valve disposed at a portion of the distribution channel connected to an outlet of the sample chamber and a second valve is disposed at a portion of the distribution channel connected to an outlet of the mixture prevention chamber.
3. The microfluidic apparatus of claim 1 , wherein the at least one valve is a normally closed valve that keeps the distribution channel closed before energy is applied thereto.
4. The microfluidic apparatus of claim 1 , wherein the at least one valve comprises a mixture of a phase transition material and a heat generation fluid.
5. The microfluidic apparatus of claim 4 , wherein the phase transition material is selected from the group consisting of wax, gel, and thermoplastic resin.
6. The microfluidic apparatus of claim 4 , wherein the heat generation fluid comprises a carrier oil and heat generation particles dispersed within the carrier oil, and wherein the heat generation particles are selected from the group consisting of metal oxides, polymer particles, quantum dots, and magnetic beads.
7. The microfluidic apparatus of claim 1 , further comprising at least one inlet channel configured to connect the distribution channel to the plurality of reaction chambers.
8. The microfluidic apparatus of claim 1 , further comprising a vent disposed at one end of the distribution channel and through which air is drained.
9. The microfluidic apparatus of claim 1 , wherein the mixture prevention material is a material that does not react with the reagent and the sample and has a density smaller than a density of water.
10. The microfluidic apparatus of claim 1 , wherein the mixture prevention material is selected from the group consisting of liquid oil, liquid paraffin wax, and silicon oil.
11. The microfluidic apparatus of claim 1 , wherein the sample is a fluid comprising a nucleic acid molecule, and the reagent is a polymerase chain reaction solution for polymerase chain reaction of the nucleic acid molecule.
12. A microfluidic system comprising:
the microfluidic apparatus of claim 1 ;
a rotary operation unit configured to rotate the microfluidic apparatus;
an energy source configured to apply energy to the at least one valve of the microfluidic apparatus from outside the microfluidic apparatus; and
a controller configured to control rotation of the rotary operation unit, thereby transporting a sample to the reaction chamber, to open the outlet of the mixture prevention chamber if the sample is transported to the reaction chamber and to transport the mixture prevention material contained in the mixture prevention chamber to the distribution channel when an outlet of the mixture prevention chamber is open.
13. The microfluidic system of claim 12 , wherein the energy source is a laser light source.
14. The microfluidic system of claim 12 , wherein the rotary operation unit is a spindle motor.
15. The microfluidic system of claim 12 , wherein movement of fluid within the microfluidic apparatus is achieved by centrifugal force that is generated as the microfluidic apparatus is rotated by the rotary operation unit.
16. The microfluidic system of claim 12 , wherein the controller is configured to, if the sample is transported to the reaction chamber, drive the rotary operation unit to move a valve disposed at the outlet of the mixture prevention chamber to a position facing the energy source, and control the energy source to apply energy to the valve, thereby opening the outlet of the mixture prevention chamber.
17. The microfluidic system of claim 12 , wherein the controller is configured to, if the outlet of the mixture prevention chamber is open, drive the rotary operation unit such that the mixture prevention material contained in the mixture prevention chamber is transported into the distribution channel.