Methamphetamine-like hapten compounds, linkers, carriers and compositions and uses thereof
The invention generally relates to hapten compounds comprising either (+) methamphetamine or (+) amphetamine conjugated to a linker. Generally speaking, hapten compounds of the invention may be used to elicit an immune response to one or more of (+) methamphetamine, (+) amphetamine, or (+) MDMA.
1. A method for generating a monoclonal antibody or an antigen binding fragment thereof, the method comprising generating the monoclonal antibody or the antigen binding fragment thereof using an immunogen comprising a hapten conjugated to an immunogenic carrier, wherein said hapten has the structure
wherein said immunogenic carrier is conjugated to said hapten via the carboxylic group of the hapten, and
wherein:
R1 is selected from the group consisting of hydrogen and methyl; and
L is selected from the group consisting of O(CH 2 ) 5 attached to the benzene ring at the meta position, and O(CH 2 ) 9 attached to the benzene ring at the meta position, wherein indicates point of attachment to the benzene ring.
2. The method of claim 1 , wherein the immunogenic carrier is selected from the group consisting of KLH, ovalbumin, BSA, cationized BSA, thyroglobulin, a bacterial toxin and a bacterial toxoid.
3. The method of claim 1 , wherein L is O(CH 2 ) 9 .
4. The method of claim 1 , wherein L is O(CH 2 ) 5 .
5. The method of claim 1 , wherein L is O(CH 2 ) 9 attached to the benzene ring at the meta position and R 1 is methyl.
6. The method of claim 1 , wherein L is O(CH 2 ) 5 attached to the benzene ring at the meta position and R 1 is methyl.