Analytical cartridge with fluid flow control
Analytical cartridges, systems and methods of processing a sample for analysis using capillary flows. Vertical gradient sample filtration provides filtrate to an incubation chamber for a time controlled by a flow modulator at the outlet of the incubation chamber. The flow modulator can include a serpentine capillary flow path without side walls. Incubated filtrate can flow from the incubation chamber to a detection channel after a predetermined time. The detection chamber can include one or more analytical regions in a porous substrate for detection of two or more analytes on the same cartridge from the same sample.
1. An analytical cartridge comprising:
a filter element comprising a sample receiving surface and a filtrate egress surface, wherein the receiving surface comprises an average pore diameter greater than an average pore diameter of the egress surface, and wherein the filter element is adapted for transverse flow and adapted so that a filtrate does not flow laterally through the filter element to the egress surface;
an incubation chamber in fluid contact with the filtrate egress surface;
a flow modulator in fluid contact with the incubation chamber; and,
a detection channel in fluid contact with the flow modulator, and comprising two or more analytical regions positioned along a surface of the channel;
whereby a flow of a filtrate from the incubation chamber is slowed by the flow modulator.
2. The cartridge of claim 1 , wherein the filter element comprises two or more filter layers comprising different average pore diameters.
3. The cartridge of claim 1 , further comprising a hydrophilic pad or hydrophilic capillary grooves in contact with the filtrate egress surface.
4. The cartridge of claim 1 , wherein a surface of the flow modulator not more hydrophobic than an outlet surface of the incubation chamber.
5. The cartridge of claim 1 , wherein the flow modulator comprises a flow path defined by opposing top and bottom path surfaces, and wherein the flow path does not comprise solid side walls.
6. The cartridge of claim 1 , further comprising a porous substrate disposed along the detection channel, which substrate comprises the analytical regions and does not fill a cross-section of the detection channel.
7. The cartridge of claim 1 , wherein the detection channel comprises a top surface and a bottom surface, and further comprises a porous substrate layer of nitrocellulose in contact with either the top surface or the bottom surface but not both surfaces.
8. The cartridge of claim 1 , wherein the detection channel has a height of less than 150 μm and the analytical regions comprise a porous substrate less than 15 μm thick in contact with a surface of the detection channel.
9. The cartridge of claim 1 , wherein the cartridge further comprises a top cover that is less hydrophilic overlying the filter element than overlying the incubation chamber.
10. The cartridge of claim 1 , wherein the detection channel is formed between a cartridge top cover and a cartridge base; and,
wherein the cover or base is transparent to interrogation by light from a detector light source.
11. A cartridge reader holding the cartridge of claim 1 and configured to detect a signal from the analytical region of the cartridge of claim 1 , wherein the reader comprises a laser with adjustable output intensity.
12. The reader of claim 11 , wherein the cartridge further comprises a bar code readable by the reader and wherein the bar code identifies a laser intensity setting.
13. The cartridge of claim 1 , wherein the analytical regions are different analytical regions.