Apparatus and method for processing biological material

1. An apparatus for concentrating cells in a suspension, the apparatus comprising:

a first chamber for receiving a suspension including a cell population, the first chamber defined by a first container, said first chamber including a cell concentration zone, said concentration zone configured to receive a concentrated cell population; and a second chamber in fluid communication with the first chamber, the second chamber defined by a second container that is physically separable from the first chamber, the second chamber including:

(i) a housing defining the second chamber as a substantially spherical inner chamber with a substantially spherical inner chamber surface;

(ii) a port in said housing communicating with the inner chamber and configured to communicate with the first chamber;

(iii) the port including a resealable closure; and

(iv) the chamber having a cell-receiving pocket positioned generally opposite said port, the cell-receiving pocket depending outwardly from the substantially spherical inner chamber surface,

said first and second chambers configured to be placed together, as a unit, in a centrifugal force field, wherein upon application of the centrifugal force field said cell-receiving pocket is configured to receive at least a portion of the concentrated cell population from the cell concentration zone of the first chamber.

2. The apparatus of claim 1 further comprising a gas collection region defined by the second chamber.

3. The apparatus of claim 2 wherein said gas collection region is adjacent to said port.

4. The apparatus of claim 1 wherein said housing is compressible.

5. The apparatus of claim 1 wherein said resealable closure is selected from the group consisting of a septum, a pre-slit septum, an elastomeric member, a gland-type valve and combinations thereof.

6. An apparatus for receiving cells in a suspension, the apparatus comprising:

a first chamber for receiving a suspension including a cell population, the first chamber defined by a first container, said first chamber including a cell concentration zone, said concentration zone configured to receive a concentrated cell population; and a second chamber in fluid communication with the first chamber, the second chamber defined by a second container that is physically separable from the first chamber, the second chamber including:

(i) a housing defining second chamber as being a substantially spherical inner chamber;

(ii) a port in the housing communicating with the inner chamber and configured to communicate with the first chamber;

(iii) the port being the only opening in the housing and including a resealable closure;

(iv) the inner chamber including a defined gas collection region comprising a volume that is located higher than a lower end of the port when the port is positioned at a top of the chamber,

said first and second chambers configured to be placed together, as a unit, in a centrifugal force field, wherein upon application of the centrifugal force field said second chamber is configured to receive at least a portion of the concentrated cell population from the cell concentration zone of the first chamber.

7. The apparatus of claim 6 wherein the housing is compressible.

8. The apparatus of claim 6 wherein the gas collection region comprises a volume that is located higher than substantially the remainder of the chamber when the port is positioned at a top of the chamber.

9. The apparatus of claim 6 wherein the gas collection region comprises at least one pocket defined in an inner surface of the chamber.

10. The apparatus of claim 6 wherein the gas collection comprises at least one pocket defined in an inner surface of the chamber and the pocket is located higher than the substantially remainder of the chamber when the port is positioned at the top of the chamber.

11. The apparatus of claim 6 in which the resealable closure is selected from the group consisting of a septum, a pre-slit septum, an elastomeric member, a gland-type valve and combinations thereof.

12. An apparatus for receiving cells in a suspension, the apparatus comprising:

a first chamber for receiving a suspension including a cell population, the first chamber defined by a first container, said first chamber including a cell concentration zone, said concentration zone configured to receive a concentrated cell population; and a second chamber in fluid communication with the first chamber, the second chamber defined by a second container that is physically separable from the first chamber, the second chamber including:

(i) a housing defining the second chamber as being an inner chamber having an inner chamber surface;

(ii) a port being the only opening in the housing communicating with the inner chamber and configured to communicate with the first chamber;

(iii) a defined cell collection region in the inner chamber surface positioned generally opposite the port, the cell collection region depending outwardly from the inner chamber surface; and

(iv) a defined gas collection region on the inner chamber surface, the gas collection region comprising a volume that is located higher than a lower end of the port when the port is positioned at a top of the chamber,

said first and second chambers configured to be placed together, as a unit, in a centrifugal force field, wherein upon application of the centrifugal force field said cell collection region is configured to receive at least a portion of the concentrated cell population from the cell concentration zone of the first chamber.

13. The apparatus of claim 12 in which the housing is compressible.

14. The apparatus of claim 12 in which the port includes a resealable closure.

15. The apparatus of claim 12 in which the chamber is generally spherical.

16. The apparatus of claim 14 in which the resealable closure is selected from the group consisting of a septum, a pre-slit septum, an elastomeric member, a gland-type valve and combinations thereof.