Methods for identifying B-clones which bind cell-surface antigens
View Patent ↗The invention provides methods for identifying immunobinders, such as scFv antibodies, capable of specifically binding to cell surface antigens, and compositions identified according to said methods.
1. A method for identifying a B-cell clone that specifically binds to an antigen of interest comprising:
(a) providing a plurality of B-cells;
(b) contacting the plurality of B-cells with the antigen of interest, wherein the antigen of interest comprises a first sortable label, and staining the plurality of B-cells with anti-IgG antibodies comprising a second sortable label and anti-IgM antibodies comprising a third sortable label;
(c) separating from the plurality of B-cells one or more B-cells that can specifically bind to the antigen of interest using a cell sorter, wherein the presence of the first and second sortable label, but not the third sortable label, in a complex is indicative of the binding of a B-cell to an antigen of interest, thereby identifying a B-cell clone that binds to the antigen of interest.
2. The method of claim 1 , wherein the plurality of B-cells is provided in a plurality of lymphocytes.
3. The method of claim 1 , wherein the plurality of B-cells are stained prior to being contacted with the antigen of interest in step (b).
4. The method of claim 1 , wherein the plurality of B-cells are stained after being contacted with the antigen of interest in step (b).
5. The method of claim 1 , wherein identification of the B-cell clone comprises clonally isolating the B-cells obtained in step (c), optionally followed by clonal expansion of the clonally isolated cells.
6. The method of claim 5 , further comprising isolating a nucleic acid molecule encoding an immunobinder from the B-cell clone that binds to an antigen of interest.
7. The method of claim 6 , further comprising producing an immunobinder capable of binding to an antigen of interest, wherein the immunobinder is produced by introducing the nucleic acid sequence into an expression environment such that the encoded immunobinder is produced.
8. The method of claim 1 , wherein the one or more separated B-cells are cultivated under suitable conditions so that antibodies are secreted into the culture medium.
9. The method of claim 8 , wherein the antibodies are tested for specific binding to the antigen of interest.
10. The method of claim 8 , wherein the antibodies are mouse, rabbit, rat, hamster, sheep, chicken, camel, goat, human antibodies.
11. The method of claim 1 , wherein the sortable labels are fluorescent labels.
12. The method of claim 1 , wherein the antigen of interest is a soluble antigen.
13. The method of claim 1 , wherein the B-cells are B-cells from a rabbit.
14. The method of claim 13 , wherein the rabbit was immunized with an antigen of interest.
15. A method for identifying a B-cell clone that specifically binds to an antigen of interest comprising:
(a) immunizing an animal with an antigen of interest;
(b) isolating a plurality of B-cells from the immunized animal;
(c) contacting the plurality of B-cells with the antigen of interest, wherein the antigen of interest comprises a first sortable label, and staining the plurality of B-cells with anti-IgG antibodies comprising a second sortable label and anti-IgM antibodies comprising a third sortable label;
(d) separating from the plurality of B-cells one or more B-cells that can specifically bind to the antigen of interest using a cell sorter, wherein the presence of the first and second sortable label, but not the third sortable label, in a complex is indicative of the binding of a B-cell to an antigen of interest, thereby identifying a B-cell clone that binds to the antigen of interest.
16. The method of claim 15 , wherein the sortable labels are fluorescent labels.
17. The method of claim 15 , wherein the antigen of interest is a soluble antigen.
18. The method of claim 15 , wherein the animal is rabbit.