Method of Producing Tissue Culture Media Derived from Plant Seed Material and Casting of Mycological Biomaterials

1 . A method of making a tissue culture comprising the steps of measuring a selected seed matter at specified ratio (m:v) with water to form a mixture;

autoclaving the mixture in an autoclavable vessel;

inoculating the autoclaved mixture with one of a cell strain and tissue strain; and

incubating the inoculated mixture for desired time to form a culture/gelling liquid.

2 . A method as set forth in claim 1 wherein said selected seed matter is selected from the group consisting of chia, flax, sesame, mustard and poppy.

3 . A method as set forth in claim 1 wherein said cell strain and tissue strain is a culture of Ganoderma applanatum.

4 . A method comprising the steps of

forming a tissue culture media composed of water, a gelling agent, at least one of seeds selected from the group consisting of chia seeds, flax, sesame, mustard and poppy and a supplemental nutrient selected from the group consisting of yeast, calcium, magnesium, complex carbohydrates and manganese;

sterilizing said media at a predetermined temperature and for a predetermined time;

cooling said sterilized media to room temperature;

inoculating said cooled media with a culture of Ganoderma applanatum to form a culture/gelling liquid;

forming a substrate composed of oat hulls, water, maltodextrin and CaSO 4 ;

sterilizing said substrate and cooling said sterilized substrate;

combining said culture/gelling liquid and said substrate to form a mixture;

placing said mixture in a mold of predetermined shape;

incubating said mixture to form a member of predetermined shape; and

drying said member to 0% moisture.

5 . A method as set forth in claim 5 wherein said culture/gelling liquid is combined with said substrate at a ratio of 20% on a volumetric basis.

6 . A method as set forth in claim 5 wherein said culture/gelling liquid is combined with said substrate at a ratio of 1 liter of culture/gelling liquid to 5 liters of substrate.

7 . A method comprising the steps of

forming a tissue culture media composed of water, a gelling agent, at least one of seeds selected from the group consisting of chia seeds, flax, sesame, mustard and poppy and a supplemental nutrient selected from the group consisting of yeast, calcium, magnesium, complex carbohydrates and manganese;

sterilizing said media at a predetermined temperature and for a predetermined time;

cooling said sterilized media to room temperature;

forming a substrate composed of oat hulls, water, maltodextrin and CaSO 4 ;

combining said culture/gelling liquid and said substrate to form a mixture;

inoculating said mixture with a Ganoderma applanatum grain inoculum;

placing said inoculated mixture in a mold of predetermined shape;

incubating said mixture to form a member of predetermined shape; and

drying said member to 0% moisture.

8 . A method as set forth in claim 7 wherein inoculum is added to said substrate at a ratio of 360 grams per 5 liters of substrate and said culture/gelling liquid is combined with said substrate at a ratio of liter of culture/gelling liquid to 5 liters of substrate.

9 . A method comprising the steps of

forming a tissue culture media composed of water, a gelling agent, at least one of seeds selected from the group consisting of chia seeds, flax, sesame, mustard and poppy and a supplemental nutrient selected from the group consisting of yeast, calcium, magnesium, complex carbohydrates and manganese;

sterilizing said media at a predetermined temperature and for a predetermined time;

cooling said sterilized media to room temperature;

forming a substrate composed of oat hulls, water, maltodextrin and CaSO 4 ;

inoculating said substrate with a culture of Ganoderma applanatum;

grinding said inoculated substrate into particles;

combining said culture/gelling liquid and said inoculated substrate to form a mixture;

placing said mixture in a mold of predetermined shape;

incubating said mixture to form a member of predetermined shape; and

drying said member to 0% moisture.

10 . A method as set forth in claim 9 wherein said culture/gelling liquid is combined with said substrate at a ratio of 1 liter of culture/gelling liquid to 5 liters of substrate.