Salivary Bioassay for Early Detection of Bone Loss
The present invention is directed to methods to detect and diagnose osteoporosis and periodontal disease using salivary biomarkers.
1 . A kit for detecting early-stage periodontal bone loss comprising a reagent that indicates the presence of a biomarker in a bodily fluid of a patient at a level that indicates early-stage periodontal bone loss and a device for contacting the bodily fluid with the biomarker.
2 . The kit of claim 1 , further comprising instructions that explains that the presence of the biomarker at the level indicates early-stage periodontal bone loss.
3 . The kit of claim 1 , wherein the bodily fluid is saliva.
4 . The kit of claim 1 , wherein the biomarker is selected from the group consisting of osteocalcin, RANK L, soluble RANKL, osteoprotegrin, alkaline phosphatase MIP 1α. 1,25 dihyroxy Vitamin D, 25-Hydroxy Vitamin D, TRAP 5b, TRACP 5b, deoxypyridinoline, FGF-23, PTH, MMP 8, MMP 13, and alpha CTX-I.
5 . The kit if claim 1 , wherein the level is greater than about 200 pg/ml.
6 . The kit if claim 1 , wherein the level is greater than about 400 pg/ml.
7 . The kit if claim 1 , wherein the level is greater than about 600 pg/ml.
8 . The kit if claim 1 , wherein the reagent is a fluorophore.
9 . A kit for detecting osteoporosis comprising a reagent that indicates the presence of a biomarker in a bodily fluid of a patient at a level that indicates osteoporosis and a device for contacting the bodily fluid with the biomarker.
10 . The kit of claim 9 , further comprising instructions that explains that the presence of the biomarker at the level indicates osteoporosis
11 . The kit of claim 9 , wherein the bodily fluid is saliva.
12 . The kit of claim 9 , wherein the biomarker is selected from the group consisting of osteocalcin, RANK L, soluble RANKL, osteoprotegrin, alkaline phosphatase MIP 1α. 1,25 dihyroxy Vitamin D, 25-Hydroxy Vitamin D, TRAP 5b, TRACP 5b, deoxypyridinoline, FGF-23, PTH, MMP 8, MMP 13, and alpha CTX-I.
13 . The kit if claim 9 , wherein the level is greater than about 200 pg/ml.
14 . The kit if claim 9 , wherein the level is greater than about 400 pg/ml.
15 . The kit if claim 9 , wherein the level is greater than about 600 pg/ml.
16 . The kit if claim 9 , wherein the reagent is a fluorophore.