Nucleic acids encoding humanized anti-CD40 antibodies
View Patent ↗Provided are humanized anti-CD40 antibodies and antigen-binding fragments and methods for treating disease characterized by expression of CD40 antigen.
1. An isolated polynucleotide encoding an antibody heavy chain variable region comprising the amino acid sequence of SEQ ID NO:10.
2. The isolated polynucleotide of claim 1 , further encoding a human IgG constant region.
3. The isolated polynucleotide or polynucleotides of claim 2 , wherein the isotype of the IgG constant region is IgG1, IgG2, IgG3, or IgG4.
4. The isolated polynucleotide or polynucleotides of claim 3 , wherein the isotype of the IgG constant region is IgG1.
5. An expression vector comprising the isolated polynucleotide of claim 1 .
6. A host cell comprising the expression vector of claim 5 .
7. The host cell of claim 6 , wherein the host cell is a mammalian host cell.
8. The host cell of claim 7 , wherein the host cell is a CHO cell.
9. An isolated polynucleotide encoding an antibody light chain variable region comprising the amino acid sequence of SEQ ID NO:16.
10. The isolated polynucleotide of claim 5 , further encoding a human IgG light chain constant region.
11. The isolated polynucleotide of claim 6 , wherein the light chain constant region is a kappa constant region.
12. An expression vector comprising the isolated polynucleotide of claim 9 .
13. A host cell comprising the expression vector of claim 12 .
14. The host cell of claim 13 , wherein the host cell is a mammalian host cell.
15. The host cell of claim 14 , wherein the host cell is a CHO cell.
16. An isolated polynucleotide or polynucleotides encoding an antibody heavy chain variable region comprising the amino acid sequence of SEQ ID NO:10 and an antibody light chain variable region comprising the amino acid sequence of SEQ ID NO:16, the heavy chain variable domain and the light chain variable domain forming an antibody or antigen binding fragment that specifically binds to human CD40.
17. An expression vector comprising the isolated polynucleotide or polynucleotides of claim 16 .
18. A host cell comprising the expression vector of claim 17 .
19. The host cell of claim 18 , wherein the host cell is a mammalian host cell.
20. The host cell of claim 19 , wherein the host cell is a CHO cell.
21. First and second vectors comprising a polynucleotide encoding an antibody heavy chain variable region comprising the amino acid sequence of SEQ ID NO:10 and a polynucleotide encoding an antibody light chain variable region comprising the amino acid sequence of SEQ ID NO:16, the heavy chain variable domain and the light chain variable domain forming an antibody or antigen binding fragment that specifically binds to human CD40.
22. A host cell comprising the vectors of claim 21 .
23. The host cell of claim 22 , wherein the host cell is a mammalian host cell.
24. The host cell of claim 23 , wherein the host cell is a CHO cell.