Electrochemical affinity biosensor system and methods
View Patent ↗The present invention provides novel osmium-based electrochemical species for the detection of wide variety of analytes using immunological techniques. The present invention also provides diagnostic kits and test sensors supporting electrode structures that can be used with the osmium-based electrochemical species. The test sensor can be fabricated to support interdigitated arrays of electrodes that have been designed to provide amplification of the electrical signal amplification desired to analyze analytes that may be present at low concentrations.
1. A compound of the formula:
wherein, R and R 1 are the same or different and each can be selected from: 2,2′-bipyridyl, 4,4′-disubstituted-2,2′-bipyridyl, 5-5′-disubstituted-2,2′-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, 5,6-disubstituted-1,10-phenanthrolinyl, or N,N′-dimethyl 2,2′-biimidazole wherein each substituent is a methyl, ethyl, or phenyl group, and where the R and R′ groups are coordinated to Os through their nitrogen atoms; R 3 is H, CH 3 or C 2 H 5 ; L is (CH 2 ) i Q, wherein i is an integer 1-10 and Q is O or NR 3 ; B is a group comprising a ligand capable of binding to a specific analyte binding partner; Z is chlorine or bromine; X is a counter ion; y is selected to provide a neutral salt; and m is 1 or 2.
2. The compound of claim 1 wherein R and R′ are the same and are selected from 2,2′-bipyridyl, 4,4′-disubstituted-2,2′-bipyridyl, or 5,5′-disubstituted-2,2′-bipyridyl substituted with a methyl, an ethyl or a phenyl group.
3. The compound of claim 1 wherein L is —(CH 2 ) n NR 3 and n is an integer between 1 and 10.
4. The compound of claim 1 wherein Q is NR 3 .
5. The compound of claim 1 wherein B comprises an epitope recognizable by an antibody capable of specific binding to an analyte.
6. The compound of claim 1 wherein B comprises an epitope capable of binding to an analyte selected from the group consisting of a biowarfare agent, an abused substance, a therapeutic agent, an environmental pollutant, a protein or a hormone.
7. The compound of claim 1 wherein X is selected from the group consisting of: chloride, bromide, iodide, and fluoride.
8. A method of detecting an analyte in a liquid sample, said method comprising:
contacting a portion of said sample with a specific binding partner for said analyte and a redox reversible conjugate, said conjugate comprising the compound of claim 1 ;
simultaneously applying a first potential voltage to a first working electrode and a second potential voltage to a second working electrode using a bipotentiostat; and
measuring a current generated by a portion of the redox reversible conjugate not bound to the specific binding partner.
9. A compound of the formula:
wherein, R and R 1 are the same or different and each can be selected from: 2,2′-bipyridyl, 4,4′-disubstituted-2,2′-bipyridyl, 5,5′-disubstituted-2,2′-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, 5,6-disubstituted-1,10-phenanthrolinyl, or N,N′-dimethyl 2, 2′-biimidazole wherein each substituent is a methyl, ethyl, or phenyl group, and where the R and R 1 groups are coordinated to Os through their nitrogen atoms, R 3 is H, CH 3 or C 2 H 5 ; L is (CH 2 ) i Q, wherein i is an integer 1-10 and Q is O or NR 3 —; A is —(CH 2 ) j —NR 3 , —(CH 2 ) j —SH, or an activated ester wherein j is an integer between 1-5; Z is chlorine or bromine; X is a counter ion; y is selected to provide a neutral salt; and m is 1 or 2.
10. The compound of claim 9 wherein A is —(CH 2 ) j —NH 2 wherein j is an integer between 1-5.