Antibodies with enhanced or suppressed effector function
Rationally designed antibodies and polypeptides that comprise multiple Fc region amino acid substitutions that synergistically provide enhanced selectivity and binding affinity to a target Fc receptor are provided. The polypeptides are mutated at multiple positions to make them more effective when incorporated in antibody therapeutics than those having wild-type Fc components.
1. A polypeptide comprising a variant Fc region, wherein said variant Fc region comprises amino acid modifications relative to a wild-type Fc region, said amino acid modifications comprising a mutation at position 239 and further comprises the mutation D265S, wherein the numbering of amino acid residues is according to the EU index as set forth in Kabat, and wherein the mutation at position 239 is S239D or S239E.
2. The polypeptide of claim 1 , wherein said modification at position 239 is S239E wherein the polypeptide has higher selectivity in binding to the FcγRIIIa receptor compared to a polypeptide comprising the wild-type Fc region.
3. The polypeptide of claim 1 , wherein said modifications further comprise the mutation S298A.
4. The polypeptide of claim 1 wherein the polypeptide comprises the amino acid modifications S239D/D265S/S298A/I332E S239E/D265S/H268D/I332E, or S239E/D265S/I332E.
5. The polypeptide of claim 1 , wherein the variant Fc region is based on a human IgG Fc region.
6. The polypeptide of claim 5 , wherein the human IgG Fc region is a human IgG1, IgG2, IgG3, or IgG4 Fc region.
7. The polypeptide of claim 1 wherein said polypeptide is an antibody.
8. The polypeptide of claim 7 , wherein said antibody is a monoclonal antibody, a humanized antibody, or a human antibody.
9. A therapeutic antibody comprising the polypeptide of claim 1 .
10. The therapeutic antibody of claim 9 , wherein said therapeutic antibody is based on a parent antibody selected from the group consisting of abagovomab, adalimumab, alemtuzumab, aurograb, bapineuzumab, basiliximab, belimumab, bevacizumab, briakinumab, canakinumab, catumaxomab, certolizumab pegol, cetuximab, daclizumab, denosumab, efalizumab, galiximab, gemtuzumab ozogamicin, golimumab, ibritumomab tiuxetan, infliximab, ipilimumab, lumiliximab, mepolizumab, motavizumab, muromonab, mycograb, natalizumab, nimotuzumab, ocrelizumab, ofatumumab, omalizumab, palivizumab, panitumumab, pertuzumab, ranibizumab, reslizumab, rituximab, teplizumab, tocilizumab/atlizumab, tositumomab, trastuzumab, VB4-847, girentuximab, ustekinumab, zalutumumab, and any other antibodies.
11. The therapeutic antibody of claim 9 , wherein said therapeutic antibody binds to a target antigen selected from the group consisting of a-chain (CD25) of IL-2R, Amyloid beta, BLyS (or BAFF), CD11a, CD20, CD22, CD23, CD3, CD4, CD52, CD80, CTLA-4, EGFR, EpCAM, F protein of RSV, G250, glycoprotein IIb/IIIa R, HER2, Hsp90, IgE antibody, IL-12/IL-23, IL-1b, IL-5, IL-6 receptor, Integrin alpha-4/beta-1, Mucin 16/CA-125, RANKL, TNF alpha, and VEGF-A.
12. A pharmaceutical composition comprising a therapeutically effective amount of the polypeptide of claim 1 , and a pharmaceutically acceptable carrier.
13. The polypeptide of claim 1 wherein the variant Fc region further comprises one or more mutations selected from the group consisting of: L234Q, L234N, L235A, G236E, E236L, E236D, G237F, G237N, S267E, S267D, S267G, H268D, H268E, E269L, E269L, D270N, D270I, D270E, S298A, K326A, K326D, A327H, A327V, A327L, A327T, A330V, A330L, A330W, A330I, A330S, I332L, I332D, and I332E.
14. The polypeptide of claim 1 wherein the variant Fc region further comprises one or more mutations selected from the group consisting of: S298A, K326A, K326D, A327H, A327V, A327L, A327T, I332L, I332D, and I332E.