Method for production of chromatography media
View Patent ↗The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D 50V for large particles/D 50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range 0.05-0.9.
1. A method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D 50V for large particles/D 50V for small particles]>3, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range of from 0.1-0.2.
2. The method of claim 1 , wherein the core is porous.
3. The method of claim 1 , wherein the core is non-porous.
4. The method of claim 1 , wherein the shell of the larger particles and the small particles are functionalized with the same type of ligand.
5. The method of claim 4 , wherein the ligands are ion exchange ligands, HIC, Chelating, protein A, Affinity or RPC ligands.
6. The method of claim 1 , wherein a porous core are functionalized with a ligand that not interact with the target molecule in a sample.
7. The method of claim 1 , wherein the shell thickness (ST) of the large beads: ST<[D 50V for small particles/2].