Preservation of cell-free RNA in blood samples
A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.
1. A composition comprising:
a. one or more nuclease inhibitors including aurintricarboxylic acid;
b. one or more preservative agents including diazolidinyl urea; and
c. two or more metabolic inhibitors including glyceraldehyde and sodium fluoride; and
d. EDTA.
2. The composition of claim 1 including from about 20% to about 30% by weight diazolidinyl urea.
3. The composition of claim 1 including from about 0.5% to about 2.5% by weight aurintricarboxylic acid.
4. The composition of claim 1 including from about 0.05% to about 1.5% by weight sodium fluoride.
5. The composition of claim 1 including from about 1% to about 8% by weight glyceraldehyde.
6. The composition of claim 1 including from about 5% to about 15% by weight EDTA.
7. The composition of claim 1 comprising from about 20% to about 30% by weight diazolidinyl urea; and from about 5% to about 15% by weight EDTA.
8. The composition of claim 7 including from about 0.5% to about 2.5% by weight of aurintricarboxylic acid.
9. The composition of claim 7 including from about 0.05% to about 1.5% by weight sodium fluoride.
10. The composition of claim 8 including from about 0.05% to about 1.5% by weight sodium fluoride.
11. The composition of claim 7 including from about 1% to about 8% by weight glyceraldehyde.
12. The composition of claim 8 including from about 1% to about 8% by weight glyceraldehyde.
13. The composition of claim 9 including from about 1% to about 8% by weight glyceraldehyde.
14. The composition of claim 10 including from about 1% to about 8% by weight glyceraldehyde.
15. A composition comprising:
a. from about 0.5% to about 2.5% by weight of aurintricarboxylic acid;
b. from about 20% to about 30% by weight diazolidinyl urea;
c. from about 0.05% to about 1.5% by Weight sodium fluoride:
d. from about 1% to about 8% by weight glyceraldehyde; and
e. from about 5% to about 15% by weight EDTA.