Granted Patent B2

US 8,883,423 · App. 13/649,839 · Granted Nov 11, 2014

Inventor: Lori A. Neely (Reading, MA)

Assignee: T2 Biosystems, Inc.

G01N27/745G01R33/448G01R33/302C12Q1/6895G01R33/465G01N24/08

Abstract

Claims (34)

1. A method for making a Candida amplicon, said method comprising amplifying by polymerase chain reaction a Candida nucleic acid from a Candida species in the presence of a forward primer and a reverse primer to produce the Candida amplicon,

wherein the forward primer consists of the oligonucleotide sequence 5′-GGC ATG CCT GTT TGA GCG TC-3′ (SEQ ID NO:1), and the reverse primer consists of the oligonucleotide sequence 5′-GCT TAT TGA TAT GCT TAA GTT CAG CGG GT-3′ (SEQ ID NO:2).

2. The method of claim 1 , wherein the Candida species is Candida albicans , and wherein the method further comprises detecting the Candida amplicon by hybridizing the amplicon with an oligonucleotide comprising a sequence selected from the group consisting of:

5′-ACC CAG CGG TTT GAG GGA GAA AC-3′ (SEQ ID NO: 3),

and

5′-AAA GTT TGA AGA TAT ACG TGG TGG ACG TTA-3′ (SEQ ID NO:4).

3. The method of claim 1 , wherein the Candida species is Candida krusei , and wherein the method further comprises detecting the Candida amplicon by hybridizing the amplicon with an oligonucleotide comprising a sequence selected from the group consisting of:

(SEQ ID NO. 5)

5′-CGC ACG CGC AAG ATG GAA ACG-3′,

(SEQ ID NO. 6)

5′-AAG TTC AGC GGG TAT TCC TAC CT-3′,

and

(SEQ ID NO. 32)

5′-AGC TTT TTG TTG TCT CGC AAC ACT CGC-3′.

4. The method of claim 1 , wherein the Candida species is Candida glabrata , and wherein the method further comprises detecting the Candida amplicon by hybridizing the amplicon with an oligonucleotide comprising a sequence selected from the group consisting of:

(SEQ ID NO: 7)

5′-CTA CCA AAC ACA ATG TGT TTG AGA AG-3′

and

(SEQ ID NO: 8)

5′-CCT GAT TTG AGG TCA AAC TTA AAG ACG TCT G-3′.

5. The method of claim 1 , wherein the Candida species is Candida parapsilosis or Candida tropicalis , and wherein the method further comprises detecting the Candida amplicon by hybridizing the amplicon with an oligonucleotide comprising a sequence selected from the group consisting of:

(SEQ ID NO: 9)

5′-AGT CCT ACC TGA TTT GAG GTCNitIndAA-3′,

(SEQ ID NO: 10)

5′-CCG NitIndGG GTT TGA GGG AGA AAT-3′,

(SEQ ID NO: 33)

5′-AAA GTT ATG AAATAA ATT GTG GTG GCC ACT AGC-3′,

(SEQ ID NO: 34)

5′-ACC CGG GGGTTT GAG GGA GAA A-3′,

(SEQ ID NO: 35)

5′-AGT CCT ACC TGA TTT GAG GTC GAA-3′,

and

(SEQ ID NO: 36)

5′-CCG AGG GTT TGA GGG AGA AAT-3′.

Assignments (2)

Continuity (9)