Mitochondrial enhancement of cells
View Patent ↗Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of stem cells. Certain embodiments disclosed herein include, but are not limited to, methods of modifying stem cells, or methods of administering modified stem cells to at least one biological tissue.
1. A method of modifying a eukaryotic cell, comprising: selecting a eukaryotic cell for modification; and
extracting mitochondria or mitochondrial DNA from a maternally genetically related platelet and modifying the selected eukaryotic cell by transferring the extracted mitochondria or mitochondrial DNA to the selected eukaryotic cell
wherein the mitochondrial DNA of the maternally genetically related cell includes fewer mutations than the endogenous mitochondrial DNA of the selected eukaryotic cell, and the selected eukaryotic cell is from a subject that is chronologically older than the subject from which the mitochondrial DNA was extracted.
2. The method of claim 1 , wherein the mitochondrial DNA is provided to the eukaryotic cell by way of at least one of endocytosis, injection or electroporation.
3. The method of claim 1 , wherein the eukaryotic cell is located in at least one of in situ, in vitro, in vivo, in utero, or ex vivo.
4. The method of claim 1 , wherein the at least one eukaryotic cell is selected for modifying based on one or more eukaryotic cell indicators, including at least one of eukaryotic cell mitochondrial quality, quantity, or arrangement, eukaryotic cell or tissue secretion, eukaryotic cell division, or eukaryotic cell cytoskeletal rearrangement.
5. The method of claim 1 , further including selecting the modified eukaryotic cell for further manipulation.
6. The method of claim 5 , wherein further manipulation includes at least one of cell membrane stripping, genetic modification, freezing, fusing with another biological cell, transplanting the modified eukaryotic cell, or implanting the modified eukaryotic cell into a recipient subject.
7. The method of claim 1 , wherein the maternally genetically related platelet is derived from at least one of the mother, biological sibling, sister's child, or mother's sister's child of the subject source of the eukaryotic cell.
8. The method of claim 7 , wherein the maternally genetically related platelet includes or is derived from at least one stem cell from at least one of the mother, biological sibling, sister's child, or mother's sister's child of the subject source of the eukaryotic cell.
9. The method of claim 1 , further including measuring the membrane potential of the selected eukaryotic cell at least one of prior to, during, or subsequent to transferring the mitochondria or mitochondrial DNA to the selected eukaryotic cell.