Method of retrovirus storage
View Patent ↗A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.
1. A method for stably storing a retrovirus, comprising:
(1) contacting a solid support on which a substance having a retrovirus-binding activity is immobilized with a retrovirus producer cell culture supernatant which contains the retrovirus;
(2) removing the retrovirus producer cell culture supernatant in step (1) from contact with the solid support;
(3) after removal of the retrovirus producer cell culture supernatant, contacting the solid support on which the retrovirus is bound via the substance having a retrovirus-binding activity immobilized on the solid support with a solution containing at least one substance selected from the group consisting of gelatin, albumin and lactose; and
(4) storing the retrovirus, bound to the solid support via the substance having a retrovirus-binding activity immobilized on the solid support obtained in step (3), in contact with the solution and in an unfrozen state for a period of 24 hours or more.
2. The method according to claim 1 , wherein the retrovirus is stored in contact with the solution so that the solution is not in contact with air.
3. The method according to claim 1 , wherein the solution contains a phosphate salt as a buffering component.
4. The method according to claim 1 , wherein one substance of the at least one substance contained in the solution is bovine serum albumin (BSA) or human serum albumin (HSA).
5. The method according to claim 1 , wherein the period of storage is 48 hours or more.
6. The method according to claim 5 , wherein the period of storage is 72 hours or more.
7. The method according to claim 1 , wherein the storing step is carried out at a temperature of 15° C. or below.
8. The method according to claim 7 , wherein the storing step is carried out at a temperature of 0-10° C.
9. The method according to claim 1 , wherein the solid support is a container selected from the group consisting of a culture plate, a culture flask, a separation bag, and a gas-permeable culture bag.