Full resolution color imaging of an object
View Patent ↗The invention relates generally to both a method and apparatus for the creation of full resolution color digital images of diagnostic cassettes or objects of interest using a gray-scale digital camera or sensor combined with time sequential illumination using additive primary colors followed by post exposure digital processing. Such procedures and equipment is of significant economic value when employed in situations such as diagnostic clinical analyzers where space is limited and image quality requirements are high.
1. A method of constructing a color digital image represented by a matrix of pixel elements or values of an object of interest comprising,
optically obtaining a red color plane of image data represented by a matrix of pixel elements or values using a gray-scale digital camera or sensor,
optically obtaining a green color plane of image data represented by a matrix of pixel elements or values using a gray-scale digital camera or sensor,
optically obtaining a blue color plane of image data represented by a matrix of pixel elements or values using a gray-scale digital camera or sensor,
multiplying, element by element, said red color plane by a red weighting matrix producing a weighted red color plane, wherein application of said red weight matrix corrects for imaging issues selected from the group consisting of non-uniform exposure, alternative exposure geometry and change in LED output intensity,
multiplying said weighted red color plane by a red gain scalar producing a final red color plane, wherein application of said red gain scalar corrects for imaging issues selected from the group variation in said gray-scale digital camera spectral response and variation in gray-scale digital camera sensitivity,
multiplying, element by element, said green color plane by a green weighting matrix producing a weighted green color plane, wherein application of said green weight matrix corrects for imaging issues selected from the group consisting of non-uniform exposure, alternative exposure geometry and change in LED output intensity,
multiplying said weighted green color plane by a green gain scalar producing a final green color plane, wherein application of said green gain scalar corrects for imaging issues selected from the group variation in gray-scale digital camera spectral response and variation in gray-scale digital camera sensitivity,
multiplying, element by element, said blue color plane by a blue weighting matrix producing a weighted blue color plane, wherein application of said blue weight matrix corrects for imaging issues selected from the group consisting of non-uniform exposure, alternative exposure geometry and change in LED output intensity,
multiplying said weighted blue color plane by a blue gain scalar producing a final blue color plane, wherein application of said blue gain scalar corrects for imaging issues selected from the group variation in gray-scale digital camera spectral response and variation in gray-scale digital camera sensitivity, and
combining said final red color plane, said final green color plane, and said final blue color plane into said color digital image of said object of interest.
2. The method of claim 1 , wherein the object of interest is a diagnostic cassette.
3. The method of claim 1 , wherein the constructed color digital image is of full resolution.
4. The method of claim 1 , wherein the color digital image of said object of interest is processed to determine the presence or absence of an agglutination reaction the processing step includes the steps of
i) separating the pixels of said color digital image into a plurality of zones,
ii) processing the numerical values of said pixels according to an algorithm to determine values for a set of variables, and
iii) processing said determined values for a set of variables to determine whether an agglutination pattern is present.