METHOD FOR ISOLATION OF SOLUBLE POLYPEPTIDES
Polypeptides with desirable biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human V H s and V L s, are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human V H s and V L s are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human V H s and V L s are also identified. The V H s and V L s identified may be used to create further libraries for identifying additional polypeptides. Further, the V H s and V L s may be subjected to DNA shuffling to select for improved biophysical properties.
1 .- 23 . (canceled)
24 . A polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO:8-54.
25 . (canceled)
26 . A nucleic acid sequence that encodes a polypeptide as claimed in claim 24 .
27 - 49 . (canceled)
50 . A multimer comprising at least two V H antibody fragments selected from SEQ ID NOs:8-22, or at least two V L antibody fragments selected from SEQ ID NOs:23-54.
51 . (canceled)
52 . A multimer comprising at least one V H antibody fragment selected from SEQ ID NOs:8-22, and at least one V L antibody fragment selected from SEQ ID NOs:23-54.
53 .- 96 . (canceled)
97 . A pharmaceutical composition comprising the polypeptide sequence of claim 24 and a pharmaceutically suitable agent.
98 .- 99 . (canceled)