Neprilysin inhibitors
In one aspect, the invention relates to compounds having the formula: where R 1 -R 6 , a, b, and Z are as defined in the specification, or a pharmaceutically acceptable salt thereof. These compounds have neprilysin inhibition activity. In another aspect, the invention relates to pharmaceutical compositions comprising such compounds; methods of using such compounds; and processes and intermediates for preparing such compounds.
1. A compound of formula I:
where:
R 1 is selected from H, —C 1-8 alkyl, —C 1-3 alkylene-C 6-10 aryl, —C 1-3 alkylene-C 1-9 heteroaryl, —C 3-7 cycloalkyl, —[(CH 2 ) 2 O] 1-3 CH 3 , —C 1-6 alkylene-OC(O)R 10 , —C 1-6 alkylene-NR 11 R 12 , —C 1-6 alkylene-C(O)R 13 , —C 0-6 alkylenemorpholinyl, —C 1-6 alkylene-SO 2 —C 1-6 alkyl,
R 10 is selected from —C 1-6 alkyl, —O—C 1-6 alkyl, —C 3-7 cycloalkyl, —O—C 3-7 cycloalkyl, phenyl, —O-phenyl, —NR 11 R 12 , —CH(R 15 )—NH 2 , —CH(R 15 )—NHC(O)O—C 1-6 alkyl, and —CH(NH 2 )CH 2 COOCH 3 ; and R 11 and R 12 are independently selected from H, —C 1-6 alkyl, and benzyl; or R 11 and R 12 are taken together as —(CH 2 ) 3-6 —, —C(O)—(CH 2 ) 3 —, or —(CH 2 ) 2 O(CH 2 ) 2 —; R 13 is selected from —O—C 1-6 alkyl, —O-benzyl, and —NR 11 R 12 ; and R 14 is —C 1-6 alkyl or —C 0-6 alkylene-C 6-10 aryl; R 15 is H, —CH 3 , —CH(CH 3 ) 2 , phenyl, or benzyl;
R 2 is —OR 21 or —CH 2 OR 21 ; and R 3 is H or —CH 3 ; where R 21 is H, —C(O)—C 1-6 alkyl, —C(O)—CH(R 22 )—NH 2 , —C(O)—CH(R 22 )—NHC(O)O—C 1-6 alkyl, or —P(O)(OR 23 ) 2 ; R 22 is H, —CH 3 , —CH(CH 3 ) 2 , phenyl, or benzyl; R 23 is H, —C 1-6 alkyl, or phenyl; or
R 2 is taken together with R 1 to form —OCR 15 R 16 — or —CH 2 O—CR 15 R 16 —, and R 3 is selected from H and —CH 3 , where R 15 and R 16 are independently selected from H, —C 1-6 alkyl, and —O—C 3-7 cycloalkyl, or R 15 and R 16 are taken together to form ═O; or
R 2 is taken together with R 3 to form —CH 2 —O—CH 2 — or —CH 2 —CH 2 —; or
R 2 and R 3 are both —CH 3 ;
Z is selected from —CH— and —N—;
R 4 is selected from H, —C 1-8 alkyl, —C 1-3 alkylene-O—C 1-8 alkyl, —C 1-3 alkylene-C 6-10 aryl, —C 1-3 alkylene-O—C 6-10 aryl, —C 1-3 alkylene-C 1-9 heteroaryl, —C 3-7 cycloalkyl, —[(CH 2 ) 2 O] 1-3 CH 3 , —C 1-6 alkylene-OC(O)R 40 , —C 1-6 alkylene-NR 41 R 42 , —C 1-6 alkylene-C(O)R 43 , —C 0-6 alkylenemorpholinyl, —C 1-6 alkylene-SO 2 —C 1-6 alkyl,
R 40 is selected from —C 1-6 alkyl, —O—C 1-6 alkyl, —C 3-7 cycloalkyl, —O—C 3-7 cycloalkyl, phenyl, —O-phenyl, —NR 41 R 42 , —CH(R 45 )—NH 2 , —CH(R 45 )—NHC(O)O—C 1-6 alkyl, and —CH(NH 2 )CH 2 COOCH 3 ; and R 41 and R 42 are independently selected from H, —C 1-6 alkyl, and benzyl; or R 41 and R 42 are taken together as —(CH 2 ) 3-6 —, —C(O)—(CH 2 ) 3 —, or —(CH 2 ) 2 O(CH 2 ) 2 —; R 43 is selected from —O—C 1-6 alkyl, —O-benzyl, and —NR 41 R 42 ; and R 44 is —C 1-6 alkyl or —C 0-6 alkylene-C 6-10 aryl; R 45 is H, —CH 3 , —CH(CH 3 ) 2 , phenyl, or benzyl;
a is 0 or 1; R 5 is selected from halo, —CH 3 , —CF 3 , and —CN;
b is 0 or an integer from 1 to 3; each R 6 is independently selected from halo, —OH, —CH 3 , —OCH 3 , —CN, and —CF 3 ;
where each alkyl group in R 1 and R 4 is optionally substituted with 1 to 8 fluoro atoms; and
where the methylene linker on the biphenyl is optionally substituted with one or two —C 1-6 alkyl groups or cyclopropyl;
or a pharmaceutically acceptable salt thereof.
2. The compound of claim 1 , where R 1 is H.
3. The compound of claim 1 , where R 1 is selected from —C 1-8 alkyl, —C 1-3 alkylene-C 6-10 aryl, —C 1-3 alkylene-C 1-9 heteroaryl, —C 3-7 cycloalkyl, —[(CH 2 ) 2 O] 1-3 CH 3 , —C 1-6 alkylene-OC(O)R 10 , —C 1-6 alkylene-NR 11 R 12 , —C 1-6 alkylene-C(O)R 13 , —C 0-6 alkylenemorpholinyl, —C 1-6 alkylene-SO 2 —C 1-6 alkyl,
or R 1 is taken together with R 2 to form —OCR 15 R 16 — or —CH 2 O—CR 15 R 16 —.
4. The compound of claim 1 , where R 1 is selected from H, —C 1-8 alkyl, —C 1-6 alkylene-OC(O)R 10 , and
where R 10 is —C 1-6 alkyl, —O—C 1-6 alkyl, or —CH[R 15 ]—NHC(O)O—C 1-6 alkyl; R 14 is —C 1-6 alkyl; R 15 is —CH(CH 3 ) 2 ; and each alkyl group in R 1 is optionally substituted with 1 to 8 fluoro atoms.
5. The compound of claim 4 , where R 1 is selected from H, —CH 2 CH 3 , —CH(CH 3 ) 2 , —CH 2 CH(CH 3 ) 2 , —(CH 2 ) 3 CH 3 , —(CH 2 ) 6 CH 3 , —CH 2 CF 3 , —(CH 2 ) 2 CF 3 , —CH 2 CF 2 CH 3 , —CH 2 CF 2 CF 3 , —CH 2 OC(O)CH 3 , —CH 2 OC(O)CH 2 CH 3 , —CH 2 OC(O)(CH 2 ) 2 CH 3 , —CH 2 OC(O)OCH 2 CH 3 , —CH 2 OC(O)—CH[CH(CH 3 ) 2 ]—NHC(O)O—CH 3 , and
where R 14 is —CH 3 .
6. The compound of claim 1 , where R 2 is —OR 21 , R 3 is H, and R 21 is H.
7. The compound of claim 1 , where R 2 is —OR 21 , R 3 is —CH 3 , and R 21 is H.
8. The compound of claim 1 , where R 2 is —CH 2 OR 21 , R 3 is H, and R 21 is H.
9. The compound of claim 1 , where R 2 is —CH 2 OR 21 , R 3 is —CH 3 , and R 21 is H.
10. The compound of claim 1 , where R 4 is H.
11. The compound of claim 1 , where R 4 is selected from —C 1-8 alkyl, —C 1-3 alkylene-O—C 1-8 alkyl, —C 1-3 alkylene-C 6-10 aryl, —C 1-3 alkylene-O—C 6-10 aryl, —C 1-3 alkylene-C 1-9 heteroaryl, —C 3-7 cycloalkyl, —[(CH 2 ) 2 O] 1-3 CH 3 , —C 1-6 alkylene-OC(O)R 40 , —C 1-6 alkylene-NR 41 R 42 , —C 1-6 alkylene-C(O)R 43 , —C 0-6 alkylenemorpholinyl, —C 1-6 alkylene-SO 2 —C 1-6 alkyl,
12. The compound of claim 1 , where R 4 is selected from H, —C 1-8 alkyl, —C 1-3 alkylene-O—C 1-8 alkyl, —C 1-3 alkylene-O—C 6-10 aryl, —[(CH 2 ) 2 O] 1-3 CH 3 , and
where R 44 is —C 1-6 alkyl; and each alkyl group in R 4 is optionally substituted with 1 to 8 fluoro atoms.
13. The compound of claim 12 , where R 4 is selected from H, —CH 2 CH 3 , —CH(CH 3 ) 2 , —CH 2 CH(CH 3 ) 2 , —(CH 2 ) 3 CH 3 , —C(CH 3 — 3 , —(CH 2 ) 2 CF 3 , —CH 2 CF 2 CH 3 , —(CH 2 ) 3 —O—CH 2 CH 3 , —(CH 2 ) 2 —O-phenyl, —(CH 2 ) 2 OCH 3 , and
where R 44 is —CH 3 .
14. The compound of claim 1 , where a is 0, or a is 1 and R 5 is halo.
15. The compound of claim 1 , where b is 0, or b is 1 and R 6 is halo, or b is 2 and each R 6 is independently selected from halo and —CH 3 .
16. The compound of claim 1 , where:
a is 0 and b is 0; or
a is 0, b is 1, and R 6 is 2′-fluoro, 3′-fluoro, 3′-chloro, or 4′-fluoro; or
a is 0, b is 2, and R 6 is 2′-fluoro, 5′-chloro or 2′-methyl, 5′-chloro or 2′,5′-dichloro; or
a is 1, R 5 is 3-chloro, and b is 0; or
a is 1, R 5 is 3-chloro, b is 1, and R 6 is 3′-chloro; or
a is 1, R 5 is 3-chloro, b is 2, and R 6 is 2′-fluoro, 5′-chloro.
17. The compound of claim 6 , where R 1 is selected from H, —C 1-8 alkyl, —C 1-6 alkylene-OC(O)R 10 , and
where R 10 is —C 1-6 alkyl, —O—C 1-6 alkyl, or —CH[R 15 ]—NHC(O)O—C 1-6 alkyl; R 14 is —C 1-6 alkyl; R 15 is —CH(CH 3 ) 2 ; and each alkyl group in R 1 is optionally substituted with 1 to 8 fluoro atoms;
Z is selected from —CH— and —N—;
R 4 is selected from H, —C 1-8 alkyl, —C 1-3 alkylene-O—C 1-8 alkyl, —C 1-3 alkylene-O—C 6-10 aryl, —[(CH 2 ) 2 O] 1-3 CH 3 , and
where R 44 is —C 1-6 alkyl; and each alkyl group in R 4 is optionally substituted with 1 to 8 fluoro atoms;
a is 0 and b is 0; or a is 0, b is 1, and R 6 is halo; or a is 0, b is 2, and one R 6 is halo and the other R 6 is halo or —CH 3 ; or a is 1, R 5 is halo, and b is 0; or a is 1, R 5 is halo, b is 1, and R 6 is halo; or a is 1, R 5 is halo, b is 2, and each R 6 is halo; and
where the methylene linker on the biphenyl is optionally substituted with two —CH 3 groups.
18. The compound of claim 17 , where R 1 is selected from H, —CH 2 CH 3 , —CH(CH 3 ) 2 , —CH 2 CH(CH 3 ) 2 , —(CH 2 ) 3 CH 3 , —(CH 2 ) 6 CH 3 , —CH 2 CF 3 , —(CH 2 ) 2 CF 3 , —CH 2 CF 2 CH 3 , —CH 2 CF 2 CF 3 , —CH 2 OC(O)CH 3 , —CH 2 OC(O)CH 2 CH 3 , —CH 2 OC(O)(CH 2 ) 2 CH 3 , —CH 2 OC(O)OCH 2 CH 3 , —CH 2 OC(O)—CH[CH(CH 3 ) 2 ]—NHC(O)O—CH 3 , and
where R 14 is —CH 3 ;
R 4 is selected from H, —CH 2 CH 3 , —CH(CH 3 ) 2 , —CH 2 CH(CH 3 ) 2 , —(CH 2 ) 3 CH 3 , —C(CH 3 ) 3 , —(CH 2 ) 2 CF 3 , —CH 2 CF 2 CH 3 , —(CH 2 ) 3 —O—CH 2 CH 3 , —(CH 2 ) 2 —O-phenyl, —(CH 2 ) 2 OCH 3 , and
where R 44 is —CH 3 ; and
a is 0 and b is 0; or a is 0, b is 1, and R 6 is 2′-fluoro, 3′-fluoro, 3′-chloro, or 4′-fluoro; or a is 0, b is 2, and R 6 is 2′-fluoro, 5′-chloro or 2′-methyl, 5′-chloro or 2′,5′-dichloro; or a is 1, R 5 is 3-chloro, and b is 0; or a is 1, R 5 is 3-chloro, b is 1, and R 6 is 3′-chloro; or a is 1, R 5 is 3-chloro, b is 2, and R 6 is 2′-fluoro, 5′-chloro.
19. The compound of claim 7 , where R 1 is H or —C 1-8 alkyl; Z is —N—; R 4 is H or —C 1-8 alkyl; and a and b are 0.
20. The compound of claim 19 , where R 1 and R 4 are H.
21. The compound of claim 8 , where R 1 is H or —C 1-8 alkyl; Z is —CH—; R 4 is H or —C 1-8 alkyl; a is 0 or a is 1 and R 5 is halo; b is 0 or b is 1 or 2 and R 6 is halo; and where the methylene linker on the biphenyl is optionally substituted with two —CH 3 groups.
22. The compound of claim 21 , where R 1 is H, —CH 2 CH 3 , or —(CH 2 ) 3 CH 3 ; R 4 is H; a is 0 or a is 1 and R 5 is 3-chloro; b is 0 or b is 1 and R 6 is 2′-fluoro, 3′-fluoro, 3′-chloro, or 4′-fluoro.
23. The compound of claim 9 , where R 1 is H or —C 1-8 alkyl; Z is —CH—; R 4 is H or —C 1-8 alkyl; a is 0; and b is 0, or b is 1 and R 6 is halo.
24. The compound of claim 23 , where R 1 is H or —CH 2 CH 3 ; R 4 is H or —CH 2 CH(CH 3 ) 2 ; and b is 0, or b is 1 and R 6 is 2′-fluoro, 3′-fluoro, 3′-chloro, or 4′-fluoro.
25. The compound of claim 1 , where R 1 is selected from H, —C 1-8 alkyl, —C 1-6 alkylene-OC(O)R 10 , and
where R 10 is —C 1-6 alkyl, —O—C 1-6 alkyl, or —CH[R 15 ]—NHC(O)O—C 1-6 alkyl; R 14 is —C 1-6 alkyl; R 15 is —CH(CH 3 ) 2 ; and each alkyl group in R 1 is optionally substituted with 1 to 8 fluoro atoms;
R 4 is selected from H, —C 1-8 alkyl, —C 1-3 alkylene-O—C 1-8 alkyl, —C 1-3 alkylene-O—C 6-10 aryl, —[(CH 2 ) 2 O] 1-3 CH 3 , and
where R 44 is —C 1-6 alkyl; and each alkyl group in R 4 is optionally substituted with 1 to 8 fluoro atoms;
a is 0 and b is 0; or a is 0, b is 1, and R 6 is 2′-fluoro, 3′-fluoro, 3′-chloro, or 4′-fluoro; or a is 0, b is 2, and R 6 is 2′-fluoro, 5′-chloro or 2′-methyl, 5′-chloro or 2′,5′-dichloro; or a is 1, R 5 is 3-chloro, and b is 0; or a is 1, R 5 is 3-chloro, b is 1, and R 6 is 3′-chloro; or a is 1, R 5 is 3-chloro, b is 2, and R 6 is 2′-fluoro, 5′-chloro; and
where the methylene linker on the biphenyl is optionally substituted with two —CH 3 groups.
26. The compound of claim 25 , where R 2 is −OR 21 or —CH 2 OR 21 ; and R 3 is H or —CH 3 ; where R 21 is H.
27. A process for preparing the compound of claim 1 , comprising the step of coupling a compound of formula 1 with a compound of formula 2:
to produce a compound of formula I; where R 1 -R 6 , a, and b are as defined in claim 1 , and P is selected from H and an amino-protecting group selected from t-butoxycarbonyl, trityl, benzyloxycarbonyl, 9-fluorenylmethoxycarbonyl, formyl, trimethylsilyl, and t-butyldimethylsilyl; and where the process further comprises deprotecting the compound of formula 1 when P is an amino protecting group.
28. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier.
29. The pharmaceutical composition of claim 28 , further comprising a therapeutic agent selected from adenosine receptor antagonists, α-adrenergic receptor antagonists, β 1 -adrenergic receptor antagonists, β 2 -adrenergic receptor agonists, dual-acting β-adrenergic receptor antagonist/α 1 -receptor antagonists, advanced glycation end product breakers, aldosterone antagonists, aldosterone synthase inhibitors, aminopeptidase N inhibitors, androgens, angiotensin-converting enzyme inhibitors and dual-acting angiotensin-converting enzyme/neprilysin inhibitors, angiotensin-converting enzyme 2 activators and stimulators, angiotensin-II vaccines, anticoagulants, anti-diabetic agents, antidiarrheal agents, anti-glaucoma agents, anti-lipid agents, antinociceptive agents, anti-thrombotic agents, AT 1 receptor antagonists and dual-acting AT 1 receptor antagonist/neprilysin inhibitors and multifunctional angiotensin receptor blockers, bradykinin receptor antagonists, calcium channel blockers, chymase inhibitors, digoxin, diuretics, dopamine agonists, endothelin converting enzyme inhibitors, endothelin receptor antagonists, HMG-CoA reductase inhibitors, estrogens, estrogen receptor agonists and/or antagonists, monoamine reuptake inhibitors, muscle relaxants, natriuretic peptides and their analogs, natriuretic peptide clearance receptor antagonists, neprilysin inhibitors, nitric oxide donors, non-steroidal anti-inflammatory agents, N-methyl d-aspartate receptor antagonists, opioid receptor agonists, phosphodiesterase inhibitors, prostaglandin analogs, prostaglandin receptor agonists, renin inhibitors, selective serotonin reuptake inhibitors, sodium channel blocker, soluble guanylate cyclase stimulators and activators, tricyclic antidepressants, vasopressin receptor antagonists, and combinations thereof.
30. The pharmaceutical composition of claim 29 , wherein the therapeutic agent is an AT 1 receptor antagonist.
31. A method for treating hypertension, heart failure, or renal disease, comprising administering to a patient a therapeutically effective amount of the compound of claim 1 .