Induction and stabilization of enzymatic activity in microorganisms
The present invention is directed to methods for inducing desired activity in enzymes or microorganisms capable of producing the enzymes. The invention is further directed to methods of stabilizing activity in microorganisms. In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase I activity. The invention further provides compositions comprising enzymes or microorganisms having induced and/or stabilized activity.
1. A method for stabilizing nitrile hydratase comprising chemically crosslinking a microorganism capable of producing the nitrile hydratase with an effective amount of glutaraldehyde such that the nitrile hydratase is sufficiently stabilized to convert nitrile compounds to their corresponding amides for at least 30 days at a temperature of at least 25° C., wherein the microorganism comprises bacteria selected from the genus Rhodococcus, genus Brevibacterium , genus Pseudomonas, genus Pseudonocardia, genus Nocardia, and combinations thereof.
2. The method of claim 1 , further comprising immobilizing the microorganism with a substrate.
3. The method of claim 2 , wherein the substrate is selected from the group consisting of alginate and amide-containing polymers.
4. The method of claim 3 , wherein the amide-containing polymer comprises polyacrylamide.