Methods of analysis of allelic imbalance
View Patent ↗Methods are provided for identification of genes that are imprinted. In another embodiment methods are provided for identification and analysis of genes whose expression shows allelic imbalance. The expression products transcribed from genes that are present in the genome as two or more alleles may be distinguished by hybridization to an array designed to interrogate individual alleles. Genes whose transcription products are present in amounts that vary from expected are candidates for allelic imbalance, imprinting and imprinting errors.
1. A method of identifying at least one heterozygous gene showing monoallelic expression in an individual comprising
assaying a genomic DNA sample from an individual to identify alleles of a heterozygous gene including a plurality of single nucleotide polymorphisms including at least 1,000 different polymorphic positions,
assaying an RNA sample from the individual to determine whether RNA is transcribed from one or more of the alleles, whereby the heterozygous gene exhibits monoallelic expression when RNA is expressed from only one of the alleles.
2. The method of claim 1 wherein the at least one heterozygous gene showing monoallelic expression is an imprinted gene.
3. The method of claim 2 wherein parental origin of the expressed allele of the imprinted gene is determined by
determining which alleles of the gene are present in the mother,
determining which alleles of the gene are present in the father, and matching the expressed allele to one of the alleles present in the mother or the father.
4. The method of claim 1 wherein the gene showing monoallelic expression is a gene encoding a lymphoid-specific factor.
5. The method of claim 1 wherein the gene showing monoallelic expression is a subunit of an olfactory receptor.
6. The method of claim 1 wherein the gene showing monoallelic expression is a subunit of a T cell receptor.
7. The method of claim 1 wherein the gene showing monoallelic expression is a subunit of an immunoglobulin.
8. The method of claim 1 wherein the genomic DNA sample and the RNA sample are differentially labeled.
9. The method of claim 1 wherein the polymorphisms are associated with a phenotype.
10. The method of claim 1 wherein the polymorphisms are associated with a disease.
11. The method of claim 10 wherein the disease is cancer.
12. The method of claim 10 wherein the disease is a neurological disorder.
13. The method of claim 1 wherein the plurality of single nucleotide polymorphisms includes at least 10,000 different polymorphic positions.
14. The method of claim 1 wherein the plurality of single nucleotide polymorphisms includes at least 100,000 different polymorphic positions.