Method and construct for synthetic bidirectional SCBV plant promoter
View Patent ↗Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
1. A double-stranded nucleic acid construct for expressing multiple genes in a plant cell and/or a plant tissue, the construct comprising a bidirectional promoter comprising:
a first strand comprising a minimal core promoter element; and
a second strand comprising a Sugar Cane Bacilloform Virus (SCBV) promoter,
wherein the bidirectional promoter comprises the nucleotide sequence of SEQ ID NO:5, wherein the minimal core promoter element drives expression of a first gene expression cassette; and wherein the SCBV promoter drives expression of a second gene expression cassette.
2. A synthetic double-stranded polynucleotide comprising:
a first strand comprising a minimal core promoter element; and
a second strand comprising a Sugar Cane Bacilliform Virus promoter,
wherein the polynucleotide comprises the sequence of SEQ ID NO:5.