Methods for producing ECM-based biomaterials
View Patent ↗A method for forming an extracellular matrix material (ECM) material includes providing at least an ECM composition containing ECM particles varying in their capacity for migration through a fluid medium, including at least one population of expanded ECM particles. The ECM composition is combined in a fluid medium to form a flowable ECM composition. The flowable ECM composition is subjected to a centrifugal force in a mold for a period of time sufficient to distribute the ECM particles according to differences in their physical characteristics. The ECM composition is dried to form a dried ECM material having a density gradient extending from a less dense region to a more dense region. The dried ECM material may formed as a porous, substantially acellular ECM material expandable in an aqueous fluid environment by at least 100% in volume.
1. An extracellular matrix (ECM) material comprising:
first and second populations of ECM particles centrifugally cast in a mold and dried to form the ECM material, the ECM material expandable in an aqueous fluid environment by at least 100% in volume,
wherein the first population is characterized by a first average particle size or first average particle density, and the second population is characterized by a second average particle size or second average particle density, wherein the corresponding average particle sizes or average particle densities differ from one another in the first and second populations; and
wherein the ECM material has a shape defined by the mold and is characterized by a density gradient extending from a less dense region to a more dense region.
2. The ECM material of claim 1 , wherein the less dense region is a central region and the more dense region is an outer region.
3. The ECM material of claim 2 , wherein the first population comprises expanded ECM particles and the second population comprises non-expanded ECM particles, and wherein the outer region comprises more non-expanded ECM particles than the inner region.
4. The ECM material of claim 3 , wherein the non-expanded ECM particles include FGF-2 native to a source tissue, and wherein the ECM material further comprises an FGF-2 gradient extending from a lower-FGF-2 content central region to a higher FGF-2 content outer region.
5. The ECM material of claim 1 , wherein the ECM material is formed into a solid plug, hollow plug or sleeve.
6. The ECM material of claim 1 , wherein the ECM material is a porous, decellularized ECM material.
7. The ECM material of claim 1 , wherein the first and second populations of ECM particles have different bioactivity relative to one another, such that the ECM material is characterized by a bioactivity gradient.
8. The ECM material of claim 1 , wherein the first and second populations of ECM particles have different levels of growth factors relative to one another.
9. The ECM material of claim 1 , wherein the first and second populations of ECM particles have different levels of glycosaminoglycans relative to one another.
10. The ECM material of claim 1 , wherein the first and second populations of ECM particles have different levels of proteoglycans relative to one another.