IP Library › Granted Patent US 8,663,933
Granted Patent B2
US 8,663,933 · App. 13/680,925 · Granted Mar 4, 2014

Recombinant protein capable of binding specifically and quickly to troponin I derived from human myocardium

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Quick Facts
Patent No.
US 8,663,933
App. No.
13/680,925
Granted
Mar 4, 2014
Kind
B2
Abstract

Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.

Claims (19)

1. A recombinant protein which binds specifically to troponin I derived from human myocardium, the recombinant protein comprising:

a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and

a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.

2. The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody.

3. The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody fragment.

4. The recombinant protein according to claim 3 , wherein the antibody fragment is a Fab antibody fragment.

5. The recombinant protein according to claim 3 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.

6. The recombinant protein according to claim 3 , wherein the antibody fragment is an scFv antibody fragment.

7. A method for binding a recombinant protein specifically to troponin I derived from human myocardium, the method comprising:

a step (a) of preparing the recombinant protein, wherein

the recombinant protein comprises:

a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and

a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65; and

a step (b) of bringing the recombinant protein into contact with the troponin I derived from human myocardium to bind the recombinant protein specifically to the troponin I derived from human myocardium.

8. The method according to claim 7 , wherein the recombinant protein is an antibody.

9. The method according to claim 7 , wherein the recombinant protein is an antibody fragment.

10. The method according to claim 9 , wherein the antibody fragment is a Fab antibody fragment.

11. The method according to claim 9 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.

12. The method according to claim 9 , wherein the antibody fragment is an scFv antibody fragment.