Antagonists of IL-6 to treat cachexia, weakness, fatigue, and/or fever
View Patent ↗The present invention is directed to therapeutic methods using antibodies and fragments thereof having binding specificity for IL-6 to prevent or treat cachexia, fever, weakness and/or fatigue in a patient in need thereof. In preferred embodiments, the anti-IL-6 antibodies will be humanized and/or will be aglycosylated. Also, in preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level. In another preferred embodiment, the patient's survivability or quality of life will preferably be improved.
1. A method of treating cachexia, weakness, fatigue, and/or fever in a patient diagnosed with an interleukin-6 (IL-6) associated disorder, comprising administering to the patient an anti-IL-6 antibody or antibody fragment, whereby the patient's cachexia, weakness, fatigue, and/or fever is improved, and monitoring the patient to assess cachexia, weakness, fatigue, and/or fever, wherein the anti-IL-6 antibody or antibody fragment comprises an anti-IL-6 antibody comprising a variable light (VL) polypeptide and a variable heavy (VH) polypeptide respectively comprising the identical CDRs as of (i) SEQ ID NO:85 and 86, or (ii) SEQ ID NO:101 and 102.
2. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment comprises V L and V H complementarity determining regions (CDRs) identical to those in V L and V H polypeptides in of (i) SEQ ID NO:85 and 86, respectively.
3. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment comprises VL chain and VH CDRs that are identical to those of SEQ ID NO:101 and 102, respectively.
4. The method of claim 3 , wherein the anti-IL-6 antibody or antibody fragment has an elimination half-life of at least about 22 days.
5. The method of claim 3 , wherein the anti-IL-6 antibody or antibody fragment comprises a human constant region.
6. The method of claim 3 , wherein the anti-IL-6 antibody or antibody fragment comprises a human IgG1 constant region.
7. The method of claim 3 , further comprising administering an antagonist of a cachexia-associated factor, weakness-associated factor, fatigue-associated factor, and/or fever-associated factor.
8. The method of claim 7 , wherein the cachexia-associated factor, weakness-associated factor, fatigue-associated factor, and/or fever-associated factor is selected from tumor necrosis factor-alpha, Interferon gamma, Interleukin 1 alpha, Interleukin 1 beta, Interleukin 6, proteolysis inducing factor, leukemia-inhibitory factor, or any combination thereof.
9. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment contains VL chain and VH polypeptides which are both at least 90% identical to (i) SEQ ID NO:85 and 86, respectively, or (ii) SEQ ID NO:101 and 102, respectively.
10. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment contains a VL chain and VH chain which are both at least 95% identical to (i) SEQ ID NO:85 and 86, respectively, or (ii) SEQ ID NO:101 and 102, respectively.
11. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment contains a VL chain and VH chain which are both identical to (i) SEQ ID NO:85 and 86, respectively, or (ii) SEQ ID NO:101 and 102, respectively.
12. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment is administered to the patient with a frequency of at most once per period of approximately four weeks.
13. The method of claim 1 , wherein at least one of the framework residues (FR residues) in said VH or VL chain polypeptides has been substituted with another amino acid residue resulting in an anti-IL-6 antibody or antibody fragment that specifically binds human IL-6.
14. The method of claim 13 , wherein one or more of said FR residues are substituted with an amino acid present at the corresponding site in a parent rabbit anti-IL-6 antibody from which the complementarity determining regions (CDRs) contained in said VH or VL polypeptides have been derived or by a conservative amino acid substitution.
15. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment has an elimination half-life of at least about 22 days.
16. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment comprises a human constant region.
17. The method of claim 1 , wherein the anti-IL-6 antibody or antibody fragment comprises a human IgG1 constant region.
18. The method of claim 1 , further comprising administering an antagonist of a cachexia-associated factor, weakness-associated factor, fatigue-associated factor, and/or fever-associated factor.
19. The method of claim 18 , wherein the cachexia-associated factor, weakness-associated factor, fatigue-associated factor, and/or fever-associated factor is selected from tumor necrosis factor-alpha, Interferon gamma, Interleukin 1 alpha, Interleukin 1 beta, Interleukin 6, proteolysis inducing factor, leukemia-inhibitory factor, or any combination thereof.