Method for expressing a mouse olfactory receptor Olfr15 on a cell membrane
View Patent ↗Provided is a method for effectively expressing mouse olfactory receptor Olfr15 on the cell membrane. The method includes steps of: bringing a cell into contact with a culture medium containing chlorpromazine; separating the culture medium from the cell so as to remove the culture medium; and incubating the cell using a culture medium which does not contain chlorpromazine to express the mouse olfactory receptor Olfr15 on the cell membrane.
1. A method for expressing a mouse olfactory receptor 15 (olfr15) on a cell membrane, the method comprising the steps of:
(a) transfecting a cell with a vector encoding the mouse Olfr15 receptor as set forth in SEQ ID NO: 20 and a receptor transporting protein,
(b) contacting the transfected cells of step (a) with a culture medium containing chlorpromazine,
(c) removing the culture medium containing chlorpromazine, and;
(d) incubating the transfected cell from step (c) in a culture medium which does not contain chlorpromazine to express the mouse Olfr15 on the cell membrane.
2. The method according to claim 1 , wherein in step (a), a vector coding for the mouse olfactory receptor Olfr15 and a vector coding for the receptor-transporting protein is used.
3. The method according to claim 1 , wherein in step (b), the concentration of the chlorpromazine is not less than 10 μg/ml and not more than 25 μg/ml.
4. The method according to claim 1 , wherein the transfected cell is left at rest between step (a) and step (b).