Clay interlaced yeast compositions and methods of utilizing the same
The present invention relates to compositions comprising yeast cells and/or yeast cell components and methods for producing and utilizing the same. In particular, the invention provides novel yeast comprising altered cell wall structure (e.g., clay and/or clay component(s) integrated (e.g., interlaced) into cell wall(s) and/or cell wall(s) comprising altered glucan:mannan ratio), methods of producing the same, compositions comprising and/or derived from the same, and methods of using the same (e.g., to sequester and/or adsorb bacteria and toxins). Compositions and methods of the invention find use in a variety of applications including dietary (e.g., admixing with feedstuffs or otherwise feeding to animals), therapeutic, prophylactic (e.g. admixing with bedding sources and/or other materials that come into contact with animals, usage during food and beverage processing and manufacture, and usage during filtration of liquids) as well as research applications.
1. A method of reducing bioavailability of mycotoxins in a feedstuff consumed by an animal or human comprising:
incorporating a clay interlaced yeast cell wall extract into the feedstuff, wherein the clay interlaced yeast cell wall extract is prepared by:
(a) cultivating yeast in the presence of clay to form a clay interlaced yeast; and
(b) extracting the clay interlaced yeast under suitable conditions to obtain the clay interlaced yeast cell wall extract.
2. The method of claim 1 , wherein about 0.0125% to about 0.4% of said clay interlaced yeast cell wall extract is added by weight to said feedstuff.
3. The method of claim 1 , wherein said animal is selected from the group consisting of avian, bovine, porcine, equine, ovine, caprine, piscines, shellfish, camelids, feline, canine and rodent species.
4. The method of claim 1 , wherein feedstuff is selected from the group consisting of a Total Mixed Ration (TMR), a forage, a pellet, a concentrate, a premix, grain, distiller grain, molasses, fiber, fodder, grass, hay, kernel, leaves, meal, distiller solubles and a feed supplement.
5. The method of claim 1 , further comprising incorporating an additional agent into said clay interlaced yeast cell wall extract incorporated feedstuff, wherein said agent is selected from the group consisting of an esterase and an epoxidase.
6. A method of feeding an animal or human a feedstuff containing a reduced bioavailablity of mycotoxin therein, the method comprising administering to the animal or human a feedstuff containing clay interlaced yeast cell wall extract, wherein the clay interlaced yeast cell wall extract is prepared by:
(a) cultivating yeast in the presence of clay to form a clay interlaced yeast; and
(b) extracting the clay interlaced yeast under suitable conditions to obtain the clay interlaced yeast cell wall extract.
7. The method of claim 6 , wherein said feedstuff selected from the group consisting of a Total Mixed Ration (TMR), a forage, a pellet, a concentrate, a premix, grain, distiller grain, molasses, fiber, fodder, grass, hay, kernel, leaves, meal, distiller solubles and a feed supplement.