Heterogeneous inseminate system
View Patent ↗A first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species of animal for the production of sex-selected embryos and sex-selected offspring.
1. An artificial heterogeneous inseminate for artificial insemination, comprising:
a first amount of sperm of a first animal; and
a second amount of sperm of a second animal combined with said first amount of sperm of said first animal, said first animal and said second animal of the same species, wherein only one of said first amount of sperm or said second amount of sperm comprises sex selected sperm, resulting from a sex selection process which separates sperm based on bearing an X-chromosome or a Y-chromosome, said sex selected sperm having a non-naturally occurring ratio of X-chromosome bearing sperm and Y-chromosome bearing sperm as compared to said second animal; wherein said first amount of sperm has sufficient concentration in said heterogeneous inseminate to increase motility of said second amount of sperm in said heterogeneous inseminate.
2. The heterogeneous inseminate of claim 1 , wherein fertility of said first amount of sperm is greater than fertility of said second amount of sperm.
3. The heterogeneous inseminate of claim 1 , wherein said first amount of sperm has a concentration in said heterogeneous inseminate effective to increase fertility of said second amount of sperm in said heterogeneous inseminate.
4. The heterogeneous inseminate of claim 1 , wherein said first amount of sperm comprises only infertile sperm.
5. The heterogeneous inseminate of claim 1 , wherein said first amount of sperm has a concentration in said heterogeneous inseminate effective to increase motility of said second amount of sperm in said heterogeneous inseminate.
6. The heterogeneous inseminate of claim 1 , wherein said sex selection process of said second amount of sperm comprises sex selection performed with a flow cytometer.
7. The heterogeneous inseminate of claim 1 , wherein said second amount of sperm in said heterogeneous inseminate comprises said sex selected sperm having a concentration of between about 2×10 4 sperm per milliliter and about 4×10 6 sperm per milliliter.
8. The heterogeneous inseminate of claim 7 , wherein said first amount of sperm in said heterogeneous inseminate has a concentration of between about 2×10 7 sperm per milliliter and about 8×10 7 sperm per milliliter.
9. The heterogeneous inseminate of claim 8 , wherein said species of animal is selected from the group consisting of: a mammal, an ayes, and a fish.
10. The heterogeneous inseminate of claim 9 , wherein said mammal comprises a bovine mammal and said heterogeneous inseminate has a dosage form of between about one-quarter milliliter and about one milliliter contained in an artificial insemination straw.
11. The heterogeneous inseminate of claim 10 , wherein said dosage form of between about one-quarter milliliter and about one milliliter contained in an artificial insemination straw comprises a frozen dosage form of between about one-quarter milliliter and about one milliliter a contained in said artificial insemination straw.
12. The heterogeneous inseminate of claim 1 , further comprising an amount of cell free seminal fluid obtained from an amount of semen containing sperm cells having greater fertility than said fertility of said first amount of sperm or said fertility of said second amount of sperm.