Sharply resolving labeled protein molecular weight standards
Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
1. A pre-labeled protein standard set comprising a plurality of labeled proteins, each protein having a known and different molecular weight ranging from 10 Da to 260 kDa, wherein one or more of said plurality of labeled proteins is a selectively labeled protein comprising a heterologous chromophore or a heterologous fluorophore label conjugated to a first amino acid, wherein said selectively labeled protein has fewer than one lysine residue per ten kilodalton (kDa) molecular weight of said selectively labeled protein, wherein at least a first selectively labeled protein comprises an amino acid sequence that has at least 80% sequence identity with the amino acid sequence of SEQ ID NO: 14.
2. The pre-labeled protein standard set of claim 1 , wherein said selectively labeled protein lacks lysine residues.
3. The pre-labeled protein standard set of claim 1 , wherein said selectively labeled protein comprises the heterologous chromophore.
4. The pre-labeled protein standard set of claim 1 , further comprising one or more proteins comprising the amino acid sequence selected from the group consisting of: SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 11, 12, 13, 15, 16, 17, 37, 38, 39, 40, and 41.