IP Library › Patent Application 13721581
Patent Application
App. No. 13/721,581

NUCLEIC ACIDS AND METHODS FOR DETECTING TURFGRASS PATHOGENIC FUNGI

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Patent No.
US None
App. No.
13/721,581
Abstract

The present invention relates to the use of at least one nucleic acid comprising or consisting of: (i) (SEQ ID NO: 1) CATCGATGAAGAACGCWGCRAAHTGCGATAMGTARTGYGAATTGCAGR ATTCAGTGARTCATCGAAWYTTTGAACGCAYMTTGCRC, wherein: R represents A or G Y represents C or T M represents A or C W represents A or T H represents A or C or T (ii) a portion of SEQ ID NO: 1, provided said nucleic acid binds under stringent conditions to a nucleic acid comprising or consisting of the complementary sequence of SEQ ID NO: 1, or (iii) complementary sequences of (i) and (ii);for the detection of nucleic acids from one or more fungi in a sample.

Claims (61)

1 . A kit for the detection of fungi, comprising at least one nucleic acid primer comprising or consisting of the sequence:

(i) CATCGATGAAGAACGCWGCRAAHTGCGATAMGTARTGYGAATTGCAG RATTCAGTGARTCATCGAAWYTTTGAACGCAYMTTGCRC (SEQ ID NO: 1), wherein:

R represents A or G

Y represents C or T

M represents A or C

W represents A or T

H represents A or C or T

(ii) a portion of SEQ ID NO: 1, provided said nucleic acid binds under stringent conditions to a nucleic acid comprising or consisting of the complementary sequence of SEQ ID NO: 1, or

(iii) a complementary sequence of (i) or (ii).

2 . The kit according to claim 1 , wherein the primer comprises or consists of:

(i) GTGARTCATCGAAWYTTTGAACGCA (SEQ ID NO: 2), wherein:

R represents A or G,

Y represents C or T,

W represents A or T,

(ii) a portion of SEQ ID NO: 2, provided said nucleic acid binds under stringent conditions to a nucleic acid comprising or consisting of the complementary sequence of SEQ ID NO: 1, or

(iii) a complementary sequence of (i) or (ii).

3 . The kit according to claim 1 , comprising one or more of the following nucleic acid sequences as the primer(s):

(SEQ ID NO: 3)

GTGAATCATCGAAACTTTGAACGCA;

(SEQ ID NO: 4)

GTGAGTCATCGAAACTTTGAACGCA;

(SEQ ID NO: 5)

GTGAATCATCGAATCTTTGAACGCA;

(SEQ ID NO: 6)

GTGAGTCATCGAATCTTTGAACGCA;

(SEQ ID NO: 7)

GTGAATCATCGAAATTTTGAACGCA;

(SEQ ID NO: 8)

GTGAGTCATCGAAATTTTGAACGCA;

(SEQ ID NO: 9)

GTGAATCATCGAATTTTTGAACGCA;

(SEQ ID NO: 10)

GTGAGTCATCGAATTTTTGAACGCA;

(SEQ ID NO: 11)

GTGAATCATCGAAACTTTGAACGCA;

(SEQ ID NO: 12)

GTGAGTCATCGAAACTTTGAACGCA;

(SEQ ID NO: 13)

GTGAATCATCGAATCTTTGAACGCA;

(SEQ ID NO: 14)

GTGAGTCATCGAATCTTTGAACGCA;

(SEQ ID NO: 15)

GTGAATCATCGAAATTTTGAACGCA;

(SEQ ID NO: 16)

GTGAGTCATCGAAATTTTGAACGCA;

(SEQ ID NO: 17)

GTGAATCATCGAATTTTTGAACGCA;

(SEQ ID NO: 18)

GTGAGTCATCGAATTTTTGAACGCA;

or their complementary sequences.

4 . The kit according to claim 1 , comprising or consisting of a sequence selected from the group consisting of SEQ ID NO: 1 to 38, or complementary sequences thereof.

5 . A nucleic acid comprising or consisting of:

(i) CATCGATGAAGAACGCWGCRAAHTGCGATAMGTARTGYGAATTGCAG RATTCAGTGARTCATCGAAWYTTTGAACGCAYMTTGCRC (SEQ ID NO: 1), wherein:

R represents A or G

Y represents C or T

M represents A or C

W represents A or T

H represents A or C or T

(ii) a portion of SEQ ID NO: 1, provided the said nucleic acid binds under stringent conditions to a nucleic acid comprising or consisting of the complementary sequence of SEQ ID NO: 1, or

(iii) a complementary sequence of (i) or (ii).

6 . A nucleic acid according to claim 5 , comprising or consisting of a sequence selected from the group consisting of SEQ ID NO: 1 to 38, or complementary sequences thereof.

Assignments (1)
CHANGE OF NAME Recorded Oct 14, 2013
From: OMYA DEVELOPMENT AG
To: OMYA INTERNATIONAL AG
Reel/Frame 031406/0917 →