Recombinant protein capable of binding specifically and quickly to troponin I derived from human myocardium
View Patent ↗Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.
1. A recombinant protein which binds specifically to troponin I derived from human myocardium, the recombinant protein comprising:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.
2. The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody.
3. The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody fragment.
4. The recombinant protein according to claim 3 , wherein the antibody fragment is a Fab antibody fragment.
5. The recombinant protein according to claim 3 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.
6. The recombinant protein according to claim 3 , wherein the antibody fragment is an scFv antibody fragment.
7. A method for binding a recombinant protein specifically to troponin I derived from human myocardium, the method comprising:
a step (a) of preparing the recombinant protein, wherein
the recombinant protein comprises:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65; and
a step (b) of bringing the recombinant protein into contact with the troponin I derived from human myocardium to bind the recombinant protein specifically to the troponin I derived from human myocardium.
8. The method according to claim 7 , wherein the recombinant protein is an antibody.
9. The method according to claim 7 , wherein the recombinant protein is an antibody fragment.
10. The method according to claim 9 , wherein the antibody fragment is a Fab antibody fragment.
11. The method according to claim 9 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.
12. The method according to claim 9 , wherein the antibody fragment is an scFv antibody fragment.