Gray leaf spot tolerant maize and methods of production
View Patent ↗The invention relates to methods and compositions for identifying maize plants that have newly conferred tolerance or enhanced tolerance to, or are susceptible to, Gray Leaf Spot (GLS). The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Maize plants that display newly conferred tolerance or enhanced tolerance to GLS that are generated by the methods of the invention are also a feature of the invention.
1. A process for identifying a corn plant with gray leaf spot tolerance, said process comprising:
(a) obtaining a genetic profile of a corn plant for a chromosomal interval on chromosome 4 between BNLG1755 and UMC1299,
(b) comparing the genetic profile to a second genetic profile of a second corn plant for the chromosomal interval on chromosome 4 between BNLG1755 and UMC1299, wherein said second genetic profile:
(i) is the genetic profile of PHJEP or a progeny of PHJEP in the chromosomal interval on chromosome 4 between BNLG1755 and UMC1299;
(ii) comprises one or more marker alleles selected from the group consisting of: allele G at PHM 00045-01, allele A at PHM 00043-01, allele A at PHM 15534-13, allele G at PHM 04694-10, allele T at PHM 01811-32, allele T at PHM 01963-15, allele C at PHM 01963-22, allele T at PHM 05013-12, allele T at PHM 00586-10, and allele A at PHM 00049-01; or
(iii) comprises one or more marker alleles selected from the group consisting of: allele G at PHM 00045-01, allele A at PHM 00043-01,allele C at PHM 01963-22, and allele T at PHM 05013-12; and
(c) identifying a corn plant as having gray leaf spot tolerance if the genetic profile of the corn plant in the chromosomal interval on chromosome 4 between BNLG1755 and UMC1299 is identical to the second genetic profile of the second corn plant in the chromosomal interval on chromosome 4 between BNLG1755 and UMC1299.
2. The process of claim 1 , wherein the first genetic profile and the second genetic profile are further defined as being for a chromosomal interval on chromosome 4 between BNLG1755 and MMC0371.