METHODS FOR PURIFYING MONOSACCHARIDE MIXTURES CONTAINING IONIC IMPURITIES
Disclosed herein are methods for separating ionic impurities from monosaccharide processing streams using simulated moving bed chromatography.
1 . A method of separating an ionic impurity from a monosaccharide-containing process stream, comprising:
a. contacting an ion exclusion resin within a simulated moving bed chromatography unit with the monosaccharide-containing process stream; and
b. eluting the ion exclusion resin with water to produce an extract stream that comprises monosaccharides and a raffinate stream that comprises the ionic impurity,
thereby separating the ionic impurity from the monosaccharide-containing process stream.
2 . A method of separating an ionic impurity from a saccharide-containing process stream, comprising:
a. providing the saccharide containing process stream, wherein the process stream further comprises an inorganic dianion;
b. contacting an ion exclusion resin within a simulated moving bed chromatography unit with the saccharide-containing process stream; and
c. eluting the ion exclusion resin with an aqueous eluent to produce an extract stream that comprises saccharides and a raffinate stream comprises the ionic impurity,
thereby separating the ionic impurity from the saccharide-containing process stream.
3 . The method of claim 1 , wherein the method is continuous.
4 . The method of claim 1 , further comprising isolating the extract steam that comprises the monosaccharides or saccharides.
5 . The method of claim 1 , further comprising isolating the raffinate stream that comprises water soluble inorganic and organic salts of sodium and ammonium.
6 . The method of claim 5 , wherein the water soluble inorganic salts of sodium and ammonium comprise sodium sulfate and ammonium sulfate.
7 . The method of claim 5 , wherein the water soluble organic salts of sodium and ammonium comprise sodium aldonate and ammonium aldonates.
8 . The method of claim 1 , wherein the monosaccharide or saccharide containing process stream comprises an L-monosaccharide.
9 . The method of claim 8 , wherein the L-monosaccharide containing process stream comprises L-mannose and L-glucose.
10 . The method of claim 1 , wherein the ion exclusion resin is a cationic exclusion resin.
11 . The method of claim 1 , wherein the ion exclusion resin is an anionic exclusion resin.
12 . The method of claim 1 , wherein the ion exclusion resin comprises a cross-linked, sulfonated polymer.
13 . The method of claim 1 , wherein the ion exclusion resin comprises a cross-linked, sulfonated polymer is in its sodium salt form.
14 . The method of claim 1 , wherein the aqueous eluent is water.
15 . The method of claim 1 , wherein the method does not comprise adding a regenerant to the ion exclusion resin.
16 . The method of claim 1 , wherein the ionic impurity comprises both an organic and inorganic impurity.
17 . The method of claim 2 , wherein the dianion is a sulfate or phosphate ion.
18 . The method of claim 1 , wherein the extract stream has a conductivity of less than about 1000 μSeimens/cm.
19 . The method of claim 1 , wherein the extract stream has a conductivity of less than about 200 μSeimens/cm.
20 . A continuous method of separating cationic and anionic impurities from a L-monosaccharide containing process stream, comprising:
a. contacting a sulfonated ion exclusion resin in its sodium salt form and within a simulated moving bed chromatography unit with the L-monosaccharide containing process stream; and
b. eluting the ion exclusion resin with water to produce an extract stream that comprises the L-monosaccharide and a raffinate stream that comprises the cationic and anionic impurities,
thereby separating the cationic and anionic impurities from the L-monosaccharide containing process stream.
21 . L-glucose having a purity of at least 98%, wherein the L-glucose is at least substantially free of the following ionic impurities:
a. (3R,4S,5S)-2,3,4,5,6-pentahydroxyhexanoate
b. (3S,4S,5S)-2,3,4,5,6-pentahydroxyhexan-1-aminium
c. CH 3 NH 3 + (methanaminium)
d. Na + (sodium),
e. NH 4 + (ammonium); and
f. SO 4 2− (sulfate).
22 . The L-glucose of claim 21 , wherein the L-glucose has a purity of at least 99.5%.
23 . The L-glucose of claim 21 , wherein the L-glucose has a conductivity of less than about 200 μSiemens/cm.
24 . L-glucose having a purity of at least 98%, wherein the L-glucose is at least substantially free of the following ionic impurities:
a. The monosodium salt of (3R,4S,5S)-2,3,4,5,6-pentahydroxyhexanoate
b. (3S,4S,5S)-2,3,4,5,6-pentahydroxyhexan-1-aminium
c. CH 3 NH 3 + (methanaminium)
d. Na 2 SO 4
e. (NH 4 ) 2 SO 4 ; and
f. H 2 Mo 7 O 24 −4 .
25 . The L-glucose of claim 24 , wherein the L-glucose has a purity of at least 99.5%.
26 . The L-glucose of claim 24 , wherein the L-glucose has a conductivity of less than about 200 μSiemens/cm.
27 . A pharmaceutical composition comprising the L-glucose of claim 21 and a pharmaceutically acceptable carrier or diluent.
28 . A method for colonic cleansing comprising administering to a subject an effective amount of the L-glucose of claim 21 .