IP Library › Patent Application 13736790
Patent Application
App. No. 13/736,790

ISOLATION OF ADULT MULTIPOTENTIAL CELLS BY TISSUE NON-SPECIFIC ALKALINE PHOSPHATASE

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Quick Facts
Patent No.
US None
App. No.
13/736,790
Abstract

The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.

Claims (29)

1 . Use of TNAP as a marker for the identification and/or enrichment of adult multipotential cells.

2 . A method of enriching for adult multipotential cells, the method comprising preparing a cell sample from a tissue source and enriching for adult multipotential cells that express the TNAP marker.

3 . A method according to claim 2 which comprises:

contacting the cell sample with a TNAP binding agent under conditions that allows binding of TNAP to the TNAP binding agent; and

separating cells bound to the TNAP binding agent.

4 - 5 . (canceled)

6 . A method according to claim 3 wherein the TNAP binding agent binds specifically to the BAP isoform of TNAP.

7 . (canceled)

8 . A method according to claim 6 wherein the anti-TNAP antibody is an antibody that binds to the same epitope as the STRO-3 antibody produced by the hybridoma cell line deposited with ATCC on 19 Dec. 2005 under the provisions of the Budapest Treaty under deposit accession number PTA-7282.

9 . (canceled)

10 . A method according to claim 8 wherein the anti-TNAP antibody is the STRO-3 antibody produced by the hybridoma cell line deposited with ATCC on 19 Dec. 2005 under the provisions of the Budapest Treaty under deposit accession number PTA-7282.

11 - 16 . (canceled)

17 . A method according to claim 2 for identifying the presence of an adult multipotential cell in a cell sample, the method comprising identifying cells in the sample that express the TNAP marker.

18 . An enriched population of adult multipotential cells obtained by a method according to claim 1 .

19 . An enriched population of TNAP+ adult multipotential cells.

20 . An expanded cell population obtained by culturing an enriched population of adult multipotential cells according to claim 18 .

21 . (canceled)

22 . A method of generating a tissue specific committed cell population, the method comprising

culturing a population of adult multipotential cells according to claim 18 in the presence of one or more stimulatory factors; and

subjecting said cultured population to conditions biasing differentiation of the adult multipotential cells to a specific tissue type.

23 - 24 . (canceled)

25 . A composition comprising a population of enriched adult multipotential cells according to claim 18 .

26 - 28 . (canceled)

29 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject an enriched or expanded cell population according to claim 18 .

30 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject a composition according to claim 25 .

31 . A method according to claim 29 wherein the tissue is bone, cardiac, or cartilage tissue.

32 - 37 . (canceled)

38 . An isolated cell which has been obtained by a method according to claim 1 , or a progeny cell thereof, wherein the cell is genetically modified.

39 - 45 . (canceled)