Oxygenated demineralized bone matrix for bone growth
View Patent ↗An improved composition for inducing bone growth is provided that is a mixture of DBM and a perfluorocarbon oxygen carrier. Injection/implantation of a composition of DBM and a perfluorocarbon results in enhancement of bone formation.
1. A non-emulsified composition, comprising a mixture of demineralized bone particles coated with a perfluorocarbon, wherein the perfluorocarbon is at or between about 32 to about 48% by weight based on the total weight of the perfluorocarbon and the DBM combined.
2. The non-emulsified composition of claim 1 , wherein the perfluorocarbon has a boiling point at or within the range of about 50° C. to about 250° C.
3. The non-emulsified composition of claim 1 , wherein the perfluorocarbon has a boiling point at or within the range of about 140° C. to about 145° C.
4. The non-emulsified composition of claim 1 , wherein the perfluorocarbon is selected from the group consisting of perfluorodecalin, perfluorohexane, perfluoroperhydrophenanthrene, perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, octafluoropropane, perfluorodichlorooctane, perfluorodecane, perfluorotripropylamine, perfluorotrimethylcyclohexane, perfluoromethyladamantane, perfluorodimethyladamantane, perfluoromethyldecaline, perfluorofluorene, diphenyldimethylsiloxane, hydrogen-rich monohydroperfluorooctane, alumina-treated perfluorooctane, and mixtures thereof.
5. The non-emulsified composition of claim 1 , wherein the perfluorocarbon is perfluorodecalin, perfluorohexane, perfluorobutylamine, perfluoroperhydrophenanthrene, or mixtures thereof.
6. The non-emulsified composition of claim 1 , wherein the perfluorocarbon is perfluorodecalin.
7. The non-emulsified composition of claim 1 , wherein the DBM is in the form of powder, granules, putty, gel, strips, paste, sheets, circular grafts, fibers, or matrices.
8. The non-emulsified composition of claim 1 , wherein the DBM is in the form of powder, granules or fibers.
9. The non-emulsified composition of claim 1 used for enhancing bone growth.
10. The non-emulsified composition of claim 1 , wherein the DBM is in the form of powder, granules, putty, gel, strips, paste, sheets, circular grafts, fibers, or matrices.
11. The non-emulsified composition of claim 1 , wherein the perfluorocarbon is perfluorodecalin, perfluorohexane, perfluorobutylamine, perfluoroperhydrophenanthrene, or combinations thereof.
12. The non-emulsified composition of claim 1 , further comprising a handling agent.
13. The non-emulsified composition of claim 12 , wherein the handling agent is selected from the group consisting of saline, glycerols, polyoxamers, lecithins, and combinations thereof.
14. A method of making a non-emulsified composition for bone growth, comprising:
coating demineralized bone matrix (DBM) particles with a perfluorocarbon to form the non-emulsified composition, the perfluorocarbon being present in the non-emulsified composition in an amount of about 32% to about 48% by weight based on the total weight of the perfluorcarbon and the DBM particles.
15. The method of claim 14 , wherein the perfluorocarbon has a boiling point at or within the range of about 50° C. to about 250° C.
16. The method of claim 14 , wherein the perfluorocarbon is selected from the group consisting of perfluorodecalin, perfluorohexane, perfluoroperhydrophenanthrene, perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, octafluoropropane, perfluorodichlorooctane, perfluorodecane, perfluorotripropylamine, perfluorotrimethylcyclohexane, perfluoromethyladamantane, perfluorodimethyladamantane, perfluoromethyldecaline, perfluorofluorene, diphenyldimethylsiloxane, hydrogen-rich monohydroperfluorooctane, alumina-treated perfluorooctane, and mixtures thereof.
17. The method of claim 14 , wherein the perfluorocarbon is perfluorodecalin.
18. The method of claim 14 , wherein DBM is in the form of powder, granules, putty, gel, strips, paste, sheets, circular grafts, fibers, or matrices.
19. The method of claim 14 , wherein the DBM is in the form of powder.
20. The method of claim 14 , further comprising adding a handling agent to the mixture.
21. The method of claim 20 , wherein the handling agent is selected from the group consisting of saline, glycerols, polyoxamers, lecithins, and combinations thereof.
22. A method of making a mixture for bone growth, comprising:
adding a perfluorocarbon directly to demineralized bone matrix (DBM) particles; and
mixing to form a mixture of DBM particles coated with the perfluorocarbon, wherein the perfluorocarbon is at or between about 32 to about 48% by weight based on the total weight of the perfluorocarbon and the DBM combined.