Placental tissue grafts
View Patent ↗Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.
1. A method for treating a separated amnion membrane comprising a first side which is an epithelial cellular layer and a second side which is a fibroblast cellular layer, which method comprises contacting the separated amnion membrane with a hypertonic saline solution, wherein the hypertonic saline solution comprises a NaCl concentration from about 10% to about 30%, under conditions such that the fibroblast cellular layer is retained.
2. The method of claim 1 , further comprising
decontaminating the separated amnion membrane in said hypertonic saline solution under agitation.
3. The method of claim 1 , further comprising substantially removing the epithelial cellular layer from the separated amnion membrane.
4. The method of claim 1 , wherein the hypertonic saline solution has a NaCl concentration of about 18%.