Syntrophic co-culture of anaerobic microorganism for production of n-butanol from syngas
This invention provides compositions for the production of butanol. Specifically, the compositions of the present invention use syntrophic co-cultures for the production of butanol from syngas.
1 . A microorganism co-culture for the conversion of at least one of CO or CO 2 and H 2 to butanol said co-culture comprising two or more microorganisms collectively having a nucleotide sequence identity at least 95% identical to SEQ ID NO: 1 and a nucleotide sequence identity at least 70% identical to SEQ ID NO: 2 or at least 65% identical to SEQ. ID NO: 3.
2 . The co-culture of claim 1 wherein the co-culture has a nucleotide sequence identity at least 75% identical to SEQ ID NO: 2.
3 . The co-culture of claim 1 wherein the co-culture has a nucleotide sequence identity at least 75% identical to SEQ ID NO: 2, and a nucleotide sequence identity at least 71% identical to SEQ. ID NO: 3.
4 . The co-culture of claim 1 wherein the co-culture includes C. kluyveri.
5 . The co-culture of claim 1 wherein the co-culture includes one or more homoacetogenic microorganisms selected from the group consisting of C. ljungdahlii, C. ragsdalei, C. authoethanongenum and C. coskatii.
6 . The co-culture of claim 5 wherein the co-culture comprises a mixture of a homoacetogenic microorganism and a butyrogenic microorganism.
7 . The co-culture of claim 6 wherein the homoacetogenic microorganism is cultured in a fermentor until it produces a concentration of ethanol of at least 1 g/L and the butyrogenic microorganism is added to the fermentor to produce the microorganism co-culture.
8 . A microorganism co-culture having a nucleotide sequence defining a gene for NADPH dependent Acetyl-CoA reductase and a nucleotide sequence defining a gene for at least one of a Butyryl-CoA acetate transferase and Butyrate kinase.
9 . The co-culture of claim 8 wherein the co-culture has a nucleotide sequence defining a gene for Butyryl-CoA acetate transferase.
10 . The co-culture of claim 9 wherein the co-culture has a nucleotide sequence defining a gene for Butyryl-CoA acetate transferase and defining a gene for Butyrate kinase.
11 . The co-culture of claim 8 wherein the co-culture includes C. kluyveri.
12 . The co-culture of claim 8 wherein the co-culture includes one or more homoacetogenic microorganisms selected from the group consisting of C. ljungdahlii, C. ragsdaeli, C. authoethanongenum and C. coskatii
13 . The co-culture of claim 12 wherein the co-culture comprises a mixture of a homoacetogenic microorganism and a butyrogenic microorganism.
14 . The co-culture of claim 13 wherein the homoacetogenic microorganism is cultured in a fermentor until it produces a concentration of ethanol of at least 10 g/L and the butyrogenic microorganism is added to the fermentor to produce the microorganism co-culture.