Boronate-mediated delivery of molecules into cells

1. A method for increasing cellular uptake of a cargo molecule by boronating the cargo molecule by binding one or more phenylboronate compounds to the cargo molecule, wherein the one or more phenylboronate compounds are selected from those of formulas:

or salts thereof,

where:

x is 1 or 2;

R 1 and R 5 are independently selected from hydrogen an optionally substituted straight-chain or branched aliphatic group having 1-8 carbon atoms a —CO 2 R 10 group, a —OCOR 10 group, a —CON(R 12 ) 2 group, a —N(R 12 ) 2 group, a —OR 10 group, a —(CH 2 ) 2 —OR 10 group, a —(CH 2 ) 2 —N(R 12 ) 2 group, a halogen, a nitro group, or a cyano group;

R 2 , R 3 , R 4 and R 6 are independently selected from hydrogen a straight-chain or branched aliphatic group having 1-8 carbon atoms, an alicyclic group, an aryl group, a heterocyclic group, a heteroaryl group, a —CO 2 R 10 group, a —OCO—R 10 group, a —CON(R 12 ) 2 hoop, a —N(R 12 ) 2 group, a —OR 10 group, a —(CH 2 ) m OH group, a halogen, a nitro group, a cyano group, -M, or two adjacent R 2 -R 6 , together with the ring carbons to which they are attached, optionally form a 5-8-member alicyclic, heterocyclic, aryl or heteroaryl ring moiety, each of which groups or moieties is optionally substituted,

wherein:

each R 10 is independently selected from hydrogen, a straight-chain or branched aliphatic group having 1-8 carbon atoms, an alicyclic group, an aryl group, a heterocyclic group, or a heteroaryl group, each of which groups is optionally substituted;

each R 12 is independently selected from hydrogen, a straight-chain or branched aliphatic group having 1-8 carbon atoms, an alicyclic group, an aryl group, a heterocyclic group, a heteroaryl group, or where two R 12 together with the nitrogen to which they are attached can form a 5-8 member heterocyclic or heteroaryl ring moiety, each of which groups or moieties is optionally substituted;

m is an integer from 1-8; and

M is a reactive group or a linker carrying a reactive group, wherein the reactive group is functional for covalent attachment of the phenylboronate to the cargo molecule and which:

(1) reacts with one or more of: an amine group, a carboxylic acid group, a sulfhydryl group or a hydroxyl group, particularly where that group is a group of a natural or unnatural amino acid of such an amino acid of a peptide or a protein;

(2) reacts with an aldehyde or ketone group, an azide group, an activated ester group, a thioester group, phosphinothioester, or other group which is introduced into or generated in the amino acid, peptide or protein; or

(3) reacts with one reactive group of a homobifunctional or a heterobifunctional crosslinking reagent,

wherein at least one of R 1 -R 6 is M and

wherein optional substitution is substitution by one or more substituents selected from halogen; a nitro group; a cyano group; a C1-C6 alkyl group; a C1-C6 alkoxy group; a C2-C6 alkenyl group; a C2-C6 alkynyl group; a 3-7 member alicyclic ring, wherein one or two ring carbons are optionally replaced with —CO— and which may contain one or two double bonds; an aryl group having 6-14 carbon ring atoms; a phenyl group; a benzyl group; a 5- or 6-member ring heterocyclic group having 1-3 heteroatoms and wherein a ring carbon is optionally replaced with —CO— and which may contain one or two double bonds; or a heteroaryl group having 1-3 heteroatoms; a —CO 2 R 13 group; —OCO—R 13 group; —CON(R 14 ) 2 group; —N(R 14 ) 2 group; or —OR 13 group, where each R 13 or R 14 is independently hydrogen; an unsubstituted C1-C6 alkyl group; an unsubstituted aryl group having 6-14 carbon atoms; an unsubstituted phenyl group; an unsubstituted benzyl group; an unsubstituted 5- or 6-member ring heterocyclic group having 1-3 heteroatoms and wherein a ring carbon is optionally replaced with —CO— and which optionally contains one or two double bonds; or a heteroaryl group having 1-3 heteroatoms and in addition two R 14 to ether with the nitro en to which the are attached optionally forms a heterocyclic or heteroaryl ring moiety, each of which groups or moieties is optionally substituted; each of which R 13 and R 14 groups is in turn optionally substituted with one or more unsubstituted C1-C3 alkyl groups, halogens, nitro groups, cyano groups, —CO 2 R 15 groups, —OCO—R 15 groups, —CON(R 16 ) 2 groups, —N(R 16 ) 2 groups, or —OR 15 groups, where each of R 15 and R 16 independently are hydrogen an unsubstituted C1-C6 alkyl group; an unsubstituted aryl group having 6-14 carbon ring atoms; an unsubstituted phenyl group; an unsubstituted benzyl group, an unsubstituted 5- or 6-member ring heterocyclic group having 1-3 heteroatoms and wherein a ring carbon is optionally replaced with —CO— and which may contain one or two double bonds; or a heteroaryl group having 1-3 heteroatoms and a total of 5-14 ring atoms; and in addition two R 16 to ether with the nitro en to which the are attached optionally form an unsubstituted heterocyclic or heteroaryl ring moiety.

2. The method of claim 1 , wherein M is a reactive group or a linker containing a reactive group wherein the reactive group reacts with one or more of: an amine, a carboxylic acid, a sulfhydryl group or a hydroxyl group.

3. The method of claim 1 , wherein M is or contains a reactive group which reacts with an aldehyde or ketone group, an azide group, an activated ester group, a thioester group, or other group which is introduced into the peptide or protein by reaction of a converting reagent with one or more of an amine group, a carboxylic acid group, a sulfhydryl group or a hydroxyl group.

4. The method of claim 1 , wherein the cargo molecule is a nucleic acid, a peptide or a protein.

5. The method of claim 1 , wherein the cargo molecule is an enzyme, an antibody or a functional fragment thereof.

6. The method of claim 1 wherein the one or more phenylboronate compounds are bound to the cargo molecule by binding of a phenylboronated oligopeptide to the peptide or protein.

7. The method of claim 6 , wherein the one or more phenylboronated oligopeptides are ligated, crosslinked or otherwise attached to the cargo molecule.

8. The method of claim 1 , wherein the one or more phenylboronate compounds are selected from those having formula:

or salts thereof.

9. The method of claim 8 , wherein R 7 and R 8 are hydrogen.

10. The method of claim 8 , wherein:

R 2 , R 3 , R 4 and R 6 are independently selected from hydrogen, a straight-chain or branched aliphatic group having 1-8 carbon atoms, an alicyclic group, an aryl group, a heterocyclic group, a heteroaryl group, a —CO 2 R 10 group, a —OCO—R 10 group, a —CON(R 12 ) 2 group, a —N(R 12 ) 2 group, a —OR 10 group, a —(CH 2 ) m OH group, a halogen, a nitro group, or a cyano group.

11. The method of claim 8 , wherein R 3 or R 4 is a —N(R 12 ) 2 group.

12. The method of claim 8 , wherein R 3 or R 4 is an —NH 2 group.

13. The method of claim 1 , wherein:

R 2 , R 3 , R 4 and R 6 are independently selected from hydrogen, a straight-chain or branched aliphatic group having 1-8 carbon atoms, an alicyclic group, an aryl group, a heterocyclic group, a heteroaryl group, a —CO 2 R 10 group, a —OCO—R 10 group, a —CON(R 12 ) 2 group, a —N(R 12 ) 2 group, a —OR 10 group, a —(CH 2 ) m OH group, a halogen, a nitro group, or a cyano group.

14. The method of claim 13 , wherein R 3 or R 4 is a —N(R 12 ) 2 group.

15. The method of claim 13 , wherein R 3 or R 4 is an —NH 2 group.

16. The method of claim 8 , wherein x is 1.

17. The method of claim 1 , wherein the one or more phenylboronate compounds retain sugar binding activity after being bound to the cargo molecule.

18. The method of claim 1 , wherein the one or more phenylboronate compounds are not bound to a polymeric material.