Polypeptides having lipase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. A detergent composition comprising an isolated polypeptide having lipase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% identity with the mature polypeptide of SEQ ID NO: 8;
(b) a polypeptide encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 7, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 7, or (iii) a full-length complementary strand of (i) or (ii), wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5× SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2× SSC, 0.2% SDS at 65° C.; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with the mature polypeptide coding sequence of SEQ ID NO: 7; a surfactant, and a stabilizing agent.
2. The detergent composition of claim 1 , wherein the polypeptide includes the amino acid sequence of SEQ ID NO: 8; or a fragment thereof having lipase activity.
3. The detergent composition of claim 1 , wherein the polypeptide includes the mature polypeptide of SEQ ID NO: 8.
4. The detergent composition of claim 1 , wherein the polypeptide consists of SEQ ID NO: 8; or a fragment thereof having lipase activity.
5. The detergent composition of claim 1 , wherein the polypeptide consists of the mature polypeptide of SEQ ID NO: 8.
6. The detergent composition of claim 1 , wherein the mature polypeptide is amino acids 19 to 348 of SEQ ID NO: 8.
7. The detergent composition of claim 1 , wherein the mature polypeptide coding sequence is nucleotides 55 to 1044 of SEQ ID NO: 7.
8. The detergent composition of claim 1 , wherein the surfactant includes one or more non-ionic surfactants.
9. The detergent composition of claim 1 , wherein the surfactant is present at a level of from 0.1% to 60% by weight.
10. The detergent composition of claim 1 , wherein the surfactant includes one or more of linear alkylbenzenesulfonate, alpha-olefinsulfonate, alkyl sulfate (fatty alcohol sulfate), alcohol ethoxysulfate, secondary alkanesulfonate, alpha-sulfo fatty acid methyl ester, alkyl- or alkenylsuccinic acid, soap, alcohol ethoxylate, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamineoxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, polyhydroxy alkyl fatty acid amide, and N-acyl N-alkyl derivatives of glucosamine (glucamides).
11. The detergent composition of claim 1 , additionally including one or more enzymes.
12. The detergent composition of claim 11 , wherein the one or more enzymes include cellulases, proteases, lipases, amylases, and peroxidases/oxidases.
13. The detergent composition of claim 1 , including a detergent builder or complexing agent.
14. The detergent composition of claim 1 , including one or more polymers.
15. The detergent composition of claim 1 , including a bleaching system.
16. The detergent composition of claim 1 , which is a liquid.
17. The detergent of claim 1 , wherein the stabilizing agent comprises a polyol, a sugar or sugar alcohol, lactic acid, boric acid, a boric acid derivative, or a phenyl boronic acid derivative.
18. The detergent of claim 1 , wherein the stabilizing agent comprises propylene glycol or glycerol.
19. The detergent of claim 1 , wherein the stabilizing agent comprises an aromatic borate ester or 4-formylphenyl boronic acid.