RECOMBINANT PROTEIN CAPABLE OF BINDING SPECIFICALLY AND QUICKLY TO TROPONIN I DERIVED FROM HUMAN MYOCARDIUM
Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.
1 . A recombinant protein which binds specifically to troponin I derived from human myocardium, the recombinant protein comprising:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.
2 . The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody.
3 . The recombinant protein according to claim 1 , wherein the recombinant protein is an antibody fragment.
4 . The recombinant protein according to claim 3 , wherein the antibody fragment is a Fab antibody fragment.
5 . The recombinant protein according to claim 3 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.
6 . The recombinant protein according to claim 3 , wherein the antibody fragment is an scFv antibody fragment.
7 . A method for binding a recombinant protein specifically to troponin I derived from human myocardium, the method comprising:
a step (a) of preparing the recombinant protein, wherein
the recombinant protein comprises:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65; and
a step (b) of bringing the recombinant protein into contact with the troponin I derived from human myocardium to bind the recombinant protein specifically to the troponin I derived from human myocardium.
8 . The method according to claim 7 , wherein the recombinant protein is an antibody.
9 . The method according to claim 7 , wherein the recombinant protein is an antibody fragment.
10 . The method according to claim 9 , wherein the antibody fragment is a Fab antibody fragment.
11 . The method according to claim 9 , wherein the antibody fragment is a F(ab′) 2 antibody fragment.
12 . The method according to claim 9 , wherein the antibody fragment is an scFv antibody fragment.