Compositions with polymerase activity
The invention provides novel compositions with polymerase activity and methods of using the compositions.
1. A polymerase that comprises a hybrid polymerase domain that has at least 94% identity to the amino acid sequence set forth in SEQ ID NO:12; or the polymerase region of SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10, wherein the hybrid polymerase domain has an increased ratio of polymerase activity to exonuclease activity relative to Pyrococcus sp. GD-B polymerase when joined to a sequence non-specific double-stranded nucleic acid binding domain.
2. The polymerase of claim 1 , wherein the hybrid polymerase domain is joined to a sequence non-specific double-stranded nucleic acid DNA binding domain, wherein the sequence non-specific double-stranded nucleic acid binding domain has at least 75% identity to the amino acid sequence set forth in SEQ ID NO:22.
3. The hybrid polymerase of claim 1 , wherein the hybrid polymerase domain is joined to a sequence non-specific double-stranded nucleic acid DNA binding domain selected from the group consisting of Sso7d, Sac7d, and Sac7e.
4. The polymerase of claim 1 , wherein the hybrid polymerase domain comprises SEQ ID NO:12.