Glyphosate resistant plants and associated methods
View Patent ↗Provided are plants having glyphosate resistance comprising a nucleic acid encoding a polypeptide having at least 90% identity with SEQ ID NO:1, and parts, organs, seeds, and/or cells of such plants. Also provided are methods for making and growing such a plant, and methods of controlling weeds in a field or area under cultivation containing such plants. For example, some methods comprise the application of multiple herbicides to such plants.
1. An isolated nucleic acid molecule comprising a polynucleotide having at least 90% sequence identity to SEQ ID NO:2 or SEQ ID NO:3,
wherein the polynucleotide encodes the polypeptide of SEQ ID NO:1, wherein the polypeptide has glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity, and
wherein the polynucleotide is operably linked to a heterologous promoter.
2. The nucleic acid molecule of claim 1 , wherein the polynucleotide is a synthetic sequence that has been designed for expression in a plant.
3. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is a vector.
4. The nucleic acid molecule of claim 3 , comprising a further polynucleotide encoding a heterologous polypeptide.
5. A host cell comprising the nucleic acid molecule of claim 1 .
6. The nucleic acid molecule of claim 1 , wherein the heterologous promoter is an AtUbi10 promoter.
7. The host cell of claim 5 , wherein the host cell is a plant cell.
8. A transgenic plant part, plant organ, or plant cell, that comprises the nucleic acid molecule of claim 1 .
9. The transgenic plant part, plant organ, or plant cell of claim 8 , wherein the plant part, plant organ, or plant cell is tolerant to glyphosate, when compared to a wild-type plant part, plant organ, or plant cell of the same species.
10. A tissue culture of regenerable plant cells that comprise the nucleic acid molecule of claim 1 .
11. A protoplast comprising the nucleic acid molecule of claim 1 .
12. The tissue culture of claim 10 , wherein the regenerable cells are selected from the group consisting of leaf cells, pollen cells, embryo cells, cotyledon cells, hypocotyl cells, meristematic cells, root cells, root tip cells, anther cells, flower cells, stem cells, and pod cells.
13. A plant or seed that comprises the nucleic acid molecule of claim 1 , wherein the plant or seed comprises the polypeptide of SEQ ID NO:1, and wherein the plant or seed is resistant to glyphosate.
14. The plant part, plant organ, or plant cell of claim 8 , wherein the polynucleotide has at least 95% sequence identity to SEQ ID NO:2 or SEQ ID NO:3, and wherein the polynucleotide and heterologous promoter are integrated in the genome of the plant part, plant organ, or plant cell.
15. A method of generating a transgenic plant part, plant organ, or plant cell, the method comprising:
transforming the plant part, plant organ, or plant cell with the nucleic acid molecule of claim 1 , so as to express the polypeptide of SEQ ID NO:1, thereby generating the transgenic plant part, plant organ, or plant cell.
16. The method according to claim 15 , wherein the plant part, plant organ, or plant cell is resistant to glyphosate.
17. The method according to claim 15 , wherein the polynucleotide has at least 95% sequence identity to SEQ ID NO:2 or SEQ ID NO:3.
18. The method according to claim 15 , wherein the polynucleotide is SEQ ID NO:2 or SEQ ID NO:3.
19. A method for controlling weeds in an area under cultivation containing the glyphosate resistant plant or seed of claim 13 , the method comprising:
applying to the area under cultivation an amount of glyphosate that controls weeds in the area under cultivation without causing more than 40% injury to the glyphosate resistant plant or seed.
20. The method according to claim 19 , wherein the glyphosate resistant plant or seed comprises a second polynucleotide encoding a heterologous polypeptide.
21. The method according to claim 20 , wherein the second polynucleotide comprises α-ketoglutarate-dependent dioxygenase-1 (aad-1) or α-ketoglutarate-dependent dioxygenase-12 (aad-12).
22. A method for conferring resistance to glyphosate in a plant, the method comprising:
transforming a plant cell with the nucleic acid molecule of claim 1 , so as to express the polypeptide of SEQ ID NO:1 in the plant; and
regenerating a transformed plant, thereby conferring resistance to glyphosate in the plant.
23. The method according to claim 22 , further comprising transforming the plant cell with a second polynucleotide encoding a heterologous polypeptide expressible in the plant.
24. The method according to claim 23 , wherein the heterologous polypeptide is α-ketoglutarate-dependent dioxygenase-1 (AAD-1) or α-ketoglutarate-dependent dioxygenase-12 (AAD-12).
25. A plant comprising the nucleic acid molecule of claim 1 in its genome, wherein the heterologous promoter is functional in the plant.
26. The plant of claim 25 , wherein the plant is a soybean plant.
27. The plant of claim 25 , wherein the plant is a corn plant.
28. The plant of claim 25 , wherein the plant is selected from the group consisting of wheat, corn, soybean, tobacco, Brachiaria , rice, millet, barley, tomato, apple, pear, strawberry, orange, alfalfa, cotton, carrot, potato, sugar beets, yam, lettuce, spinach, petunia, rose, chrysanthemum, turf grass, pine, fir, spruce, heavy metal accumulating plants, sunflower, safflower, rapeseed, and Arabidopsis.
29. The plant of claim 25 , wherein the plant is a species selected from the group consisting of genera Asparagus, Avena, Brachiaria, Brassica, Citrus, Citrullus, Capsicum, Cucurbita, Daucus, Erigeron, Glycine, Gossypium, Hordeum, Helianthus, Lactuca, Lolium, Lycopersicon, Malta, Manihot, Nicotiana, Orychophragmus, Oryza, Persea, Phaseolus, Pisum, Pyrus, Prunus, Raphanus, Secale, Solanum, Sorghum, Triticum, Vitis, Vigna , and Zea.
30. The method according to claim 16 , further comprising regenerating a plant that is resistant to glyphosate.
31. The method according to claim 19 , wherein the glyphosate is applied to the area under cultivation in an amount of between 420 g ae/ha and 3360 g ae/ha.
32. The glyphosate-resistant plant or seed of claim 13 , wherein the plant or seed is a plant.