Multiple time windows with associated calibration curves for extending the range of an assay
View Patent ↗Disclosed is a use of reaction kinetics to generate multiple dose-response curves from a single reaction, thus eliminating the need to run a second experiment with additional sample, reagents, and time to cover a broader measuring range than is available in a standard assay. Using a single protocol, the differences in the reaction kinetics for different sample concentrations yield different responses at different measurement times. Selection of the appropriate dose-response curve cross-section increases the measuring range and accuracy of the assay from a single reaction without substantially increasing imprecision. Several overlapping dose-response curves are pieced together to provide a standard curve to ensure continuity throughout the expanded measuring range.
1. A method for extending the range of a single assay and for measuring an analyte level of the assay based on a time-variant signal reflecting a dynamic response level in an instrument, the method comprising:
using a first calibration curve representative of analyte level and response level of the assay at a first time point subsequent to initiation of the assay to generate the analyte level if a signal strength corresponds to a predefined signal level for the first time point; and
if the signal strength fails to correspond to the predefined signal level for the first time point, then using a second calibration curve representative of analyte level and response level of the assay at a second time point subsequent to the first time point to estimate the analyte level if the signal strength corresponds to a predefined signal level for the second time point.
2. A method for measuring a level of an analyte using a plurality of calibration curves, each calibration curve associated with at least one threshold in regard to a single reaction at a corresponding plurality of fixed time points after initiation of the single reaction, the method comprising:
determining whether a first condition is met based on comparing a measured signal level with a first predetermined threshold associated with a first calibration curve from the plurality of calibration curves is satisfied at a first time point subsequent to initiation of the reaction, in which the first calibration curve is representative of signal levels and corresponding analyte levels at the first time point, and if the first condition is satisfied then using the first calibration curve to generate a first measured value for the level of the analyte; and
determining whether a second condition is met based on comparing a measured signal level at a second time of the reaction with a second predetermined threshold associated with a second calibration curve from the plurality of calibration curves at the second time point.
3. The method of claim 2 , wherein if more than one calibration curve from the plurality of calibration curves are available for measuring the level of the analyte, due to thresholds corresponding to each of the more than one calibration curve being satisfied at corresponding time points of the single reaction, then using an average of analyte levels corresponding to each of the available calibration curves as the measured level of the analyte.
4. The method of claim 3 , wherein the average is a weighted average of the analyte levels of the available calibration curves.
5. The method of claim 2 , wherein the first condition requires that a signal corresponding to the level of the analyte exceed the first threshold.
6. The method of claim 2 , wherein the first condition requires that a signal corresponding to the level of the analyte be less than the first threshold.
7. The method of claim 2 , wherein the first condition requires that a signal corresponding to the level of the analyte be equal to the first threshold.
8. The method of claim 2 , wherein the second condition requires that a signal corresponding to the level of the analyte exceed the second threshold.
9. The method of claim 2 , wherein the second condition requires that a signal corresponding to the level of the analyte be less than the second threshold.
10. The method of claim 2 , wherein the second condition requires that a signal corresponding to the level of the analyte be equal to the second threshold.
11. A method for scheduling a test in a clinical analyzer supporting an extended range, the method comprising the steps of:
initiating the test using a reaction mix;
determining a first signal strength from the reaction mix at a first predefined time point after initiating the test;
determining whether there is a suitable first calibration curve corresponding to the first predefined time point by comparing the determined first signal strength with a first predetermined threshold signal level of the first calibration curve, wherein the first calibration curve is suitable if the determined first signal strength at least equals the first predetermined threshold signal level;
scheduling, if there is no suitable first calibration curve, a second predefined time point after initiating the test for determining a second signal strength from the reaction mix;
determining, at the second predefined time point, the second signal strength;
identifying a second calibration curve corresponding to the second predefined time point and determining if the second calibration curve is suitable by comparing the determined second signal strength with a second predetermined threshold signal level of the second calibration curve; and
determining a level of an analyte from one or more of the first signal strength and second signal strength.
12. A diagnostic clinical analyzer supporting an extended range comprising a scheduler for implementing the reading of a signal at a time point following initiation of a single assay, the time point of the assay having a corresponding calibration curve selected from a plurality of calibration curves, wherein a determination is made by the analyzer as to the suitability of at least one said calibration curve based on the read signal as compared to a threshold signal of the corresponding calibration curve at time points of the assay.
13. A method for scheduling a test in a clinical analyzer supporting an extended range, the method comprising the steps of:
initiating the test using a reaction mix;
measuring a signal strength from the reaction mix at a first time point subsequent to the initiation of the test selected from a plurality of time points subsequent to initiating the test;
determining analyte level using a first calibration curve corresponding to the first time point for measuring the signal strength, the first calibration curve being representative of signal strength corresponding to analyte values at the first time point and in which the measured signal strength is compared to a predetermined threshold signal strength value;
scheduling, if there is no corresponding calibration curve, a second time point subsequent to the initiation of the test for determining a second signal strength from the reaction mix; and
determining analyte level using a second calibration curve corresponding to the second time point for measuring the signal strength.
14. The test scheduling method of claim 13 further comprising the step of scheduling multiple time points subsequent to the initiation of the test for measuring analyte levels using corresponding calibration curves.
15. The test scheduling method of claim 14 , wherein a reported analyte level is a mean of measured analyte levels.
16. The test scheduling method of claim 15 , wherein the reported analyte level is a weighted mean of measured analyte levels.