Systems and methods for sorting particles
View Patent ↗A flow cytometry system and method for sorting a mixture of particles, such as a fluid switching sorting system for sorting selected particles which includes a control having a microprocessor programmed with instructions for implementing a control strategy for controlling the fluid delivery to the system to vary the rate at which fluid is delivered as a function of the purity or yield of a sub-population of particles.
1. A flow cytometry system for sorting a mixture of particles including particles having a characteristic A and particles having a characteristic B, the system including:
a fluid delivery system for delivering a fluid containing the particles to a flow cytometry apparatus;
a flow cytometry apparatus for receiving the fluid, forming the fluid into a stream and using flow cytometry to classify the particles according to the characteristics; and
a fluid switching sorting system for sorting selected particles in the stream according to the classification and according to a sorting strategy to provide at least one population containing desired particles, wherein the fluid switching system comprises:
a control having a microprocessor programmed with instructions for implementing a control strategy responsive to information received from the flow cytometry apparatus during sorting for controlling the fluid delivery system during sorting to vary a rate at which fluid is delivered as a function of at least one of the following:
(1) a purity of the said at least one population with respect to either characteristic A particles or characteristic B particles; and
(2) a quantity of characteristic A particles or characteristic B particles in the said at least one population relative to a total quantity of characteristic A particles or characteristic B particles in the stream.
2. The system as claimed in claim 1 , wherein the particle are sperm cells, and wherein characteristic A is indicative of a live X sperm cell and characteristic B is indicative of something other than a live X cell (˜X).
3. The system as claimed in claim 1 ,wherein the control is suitable for determining the purity based on output signals from the flow cytometry apparatus, the system further comprises an operator input to the control for indicating a desired purity, and wherein the control varies the rate of fluid delivery to obtain the desired purity.
4. The system as claimed in claim 1 , wherein the particles within the stream arrive in a series at the flow cytometry apparatus which includes sequential sets of particles, including:
first particle sets each including one or more particles having characteristic A;
second particle sets each including one or more particles having characteristic B; and
third particle sets each including two or more closely spaced particles at least one of which has characteristic A and at least one of which has characteristic B.
5. The system as claimed in claim 4 , wherein the fluid switching sorting system diverts either only the second particle sets or the second particle sets and the third particle sets in the stream.
6. The system as claimed in claim 4 , wherein the fluid switching sorting system diverts only the second particle sets and the control maintains the purity of the said at least one population at more than 85% but less than 95%.
7. The system as claimed in claim 4 , wherein the fluid switching sorting system diverts the second particle sets and the third particle sets in the stream and wherein the control increases the rate of fluid delivery when the quantity of characteristic A particles in the said at least one population is greater than an acceptable quantity of characteristic A particles in the said at least one population, and wherein the control decreases the rate of fluid delivery when the quantity of characteristic A particles in the said at least one population is less than the acceptable quantity.
8. The system as claimed in claim 4 , wherein the fluid switching sorting system diverts the second particle sets and the third particle sets in the stream and wherein the control determines the quantity of characteristic A particles in the said at least one population based on output signals from the flow cytometry apparatus, the system further including an operator input to the control for indicating an acceptable quantity of characteristic A particles in the said at least one population, and wherein the control varies the fluid delivery rate to obtain the acceptable quantity of characteristic A particles in the said at least one population.
9. A method of sorting a mixture of particles including particles having a characteristic A and particles having a characteristic B comprising:
providing a flow cytometry system;
delivering a fluid containing the particles to a flow cytometry apparatus;
forming the fluid into a stream and using flow cytometry to classify the particles in the stream according to the characteristics;
sorting the particles in the stream by diverting selected particles in the stream according to the classification and according to a sorting strategy thereby to provide at least one population containing desired particles; and
during sorting varying a rate at which fluid is delivered as a function of at least one of the following:
(1) a purity of the said at least one population with respect to either characteristic A particles or characteristic B particles; and
(2) a quantity of characteristic A particles or characteristic B particles in the said at least one population relative to a total quantity of characteristic A particles or characteristic B particles in the stream.
10. The method as claimed in claim 9 , wherein the particles are sperm cells, and wherein characteristic A is indicative of a live X sperm cell.
11. The method as claimed in claim 9 , further comprising the step of increasing the rate of fluid delivery when the purity of the said at least one population is greater than a desired purity and decreasing the rate of fluid delivery when the purity is less than the desired purity.
12. The method as claimed in claim 9 , wherein the fluid stream contains particles following one after another in a series including sequential sets of particles, wherein the sequential sets of particles comprise:
first particle sets each including one or more particles having characteristic A;
second particle sets each including one or more particles having characteristic B; and
third particle sets each including two or more closely spaced particles at least one of which has characteristic A and at least one of which has characteristic B.
13. The method as claimed in claim 12 , further comprising the step of diverting either the second particles sets and not the first and third particle sets or the second and third particle sets and not the first particle sets.
14. The method as claimed in claim 12 , further comprising the step of diverting the second particles sets and not the first and third particle sets and further includes maintaining the purity of the said at least one population at more than 85% but less than 95%.
15. The method as claimed in claim 12 , further comprising the step of diverting the second and third particle sets and not the first particle sets and further includes increasing the rate of fluid delivery when the quantity of characteristic A particles in the said at least one population is greater than an acceptable quantity of characteristic A particles in the said at least one population, and decreasing the rate of fluid delivery when the quantity of characteristic A particles in the said at least one population is less than the acceptable quantity.