Integrated high throughput system for the analysis of biomolecules

1. An affinity microcolumn comprising a porous molecular trap having a high surface area material comprising a polymer, which has high flow properties and a low dead volume, contained within a housing and affinity reagents bound to the surface of the high surface area material, wherein the affinity reagents are either activated or activatable and the high surface area is formed by chemical etching.

2. The affinity microcolumn of claim 1 wherein the porous molecular trap is formed by molding.

3. The affinity microcolumn of claim 1 wherein the affinity reagents that are bound to the surface of the high surface area material further comprise affinity receptors bound to the affinity reagents.

4. The affinity microcolumn of claim 3 further comprising a tethering molecule that is activated or activatible and binds the affinity receptors to the affinity reagents.

5. The affinity microcolumn of claim 3 further comprising an amplification media that is activated or activatible and is interposed between the affinity reagents and the affinity receptors, where the amplification media allows a high density of affinity receptors to be bound to the affinity reagents than in the absence of the amplification media.

6. The affinity microcolumn of claim 3 further comprising an amplification media interposed between the affinity reagents and the affinity receptors, where the amplification media allows better access by an analyte to the affinity receptors than in the absence of the amplification media.

7. The affinity microcolumn of claim 5 wherein the amplification media comprises a non-biological organic polymer.

8. The affinity microcolumn of claim 6 wherein the amplification media comprises a non-biological organic polymer.

9. The affinity microcolumn of claim 1 wherein the housing is a micropipette.

10. The affinity microcolumn of claim 9 wherein the housing further comprises at least one projection that supports the porous molecular trap.